Abstract: Microorganisms are genetically engineered to continuously co-produce amino acids, high-value, specialized proteins, microbial biomass, chemicals, or any combination thereof by microbial fermentation, particularly by microbial fermentation of a gaseous substrate. The microorganisms are C1-fixing. The production of ethylene, microbial biomass, and heterologous high-value, specialized proteins can be improved. This can be improved by varying promoters or nutrient limiting means.

Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

Abstract: Integrated process for providing multiple raw materials to a downstream operation to generate an article of manufacture. Pyrolysis, torrefaction, reforming, partial oxidation, and/or gasification is integrated with gas fermentation. Integrated process converts carbon sources that would otherwise be vented to the atmosphere or discarded as waste to a fermentation product and one or more byproducts, which in turn are provided to a downstream operation to generate an article of manufacture. In certain aspects, the fermentation product may be ethanol, isoprene, butane diol, ethylene. In certain aspects, the byproduct may be metal, metal oxide, char, silica, and or steel. The downstream operation to generate an article of manufacture may be an operation to generate plastic products, rubber products, textiles, clothes, and the like.

Abstract: The systems and methods disclosed herein provide for the efficient separation of gas from fluid comprising liquid. In particular, gas separator systems and methods for use in bioreactor systems and operations are disclosed herein providing an efficient means to remove gaseous waste products, unreacted gaseous substrates, and or inert gas from broth of the biological fermentation of carbon substrates using a microorganism biocatalyst.

Abstract: Systems and methods relating to dynamic spargers for generating fine bubbles within reactors such as biological and chemical reactors. A sparger system is positioned within a reactor and comprises a support plate, multiple annular shrouds engaged with the support plate, and spargers positioned within the annular shrouds defining a gap between an interior surface of the annular shroud and an exterior surface of the corresponding sparger. Liquid flows through the defined gap between an interior surface of the annular shroud and an exterior surface of the sparger. Acceleration of the liquid through the gap shears bubbles at the exterior surface of the sparger creating bubbles or fine bubbles.

Abstract: The disclosure is directed to an apparatus and method for recovering ethanol from a fermentation broth. The fermentation broth comprises microbial biomass, ethanol, methanol, ethyl acetate, at least one thiol, and at least one compound having 3 or more carbon atoms. The method comprises separating at least microbial biomass from the fermentation broth to generate a process stream; removing, in any order, from the process stream: ethyl acetate by reacting ethyl acetate with a base compound followed by distillation; at least one thiol by adsorption or reaction to disulfide; methanol by distillation; compounds having 3 or more carbon atoms by distillation; and recovering ethanol by distillation; wherein the distillations may be conducted in a single column or two or more columns.

Abstract: The disclosure is directed to a process and an apparatus for providing a feedstock. A gaseous feed stream comprising at least one hydrocarbon is passed to a reforming unit followed by a water gas shift reaction zone to provide a first gaseous stream comprising H2, CO, and CO2. The first gaseous stream is fed a hydrogen separation zone to separate it into a hydrogen enriched stream and a second gaseous stream comprising CO, CO2 and H2. The second gaseous stream is fed to a CO2 to CO conversion system to produce a third gaseous stream comprising H2 and CO having a H2:CO molar ratio of less than 5:1. The third gaseous stream is fed as the feedstock for a gas fermentation unit to have increased stability and product selectivity.

Abstract: Provided herein is a genetically engineered microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus. Replacement of the acetolactate decarboxylase gene with DNA encoding one or more native or nonnative enzymes confers certain advantages, including fermentation stability and increased production of native and nonnative products from gaseous substrates.

Abstract: The invention present provides a method (and suitable apparatus) to convert biomass to ethanol, comprising gasifying the biomass to produce raw syngas; feeding the raw syngas to an acid-gas removal unit to remove at least some CO2 and produce a conditioned syngas stream; feeding the conditioned syngas stream to a fermentor to biologically convert the syngas to ethanol; capturing a tail gas from an exit of the fermentor, wherein the tail gas comprises at least CO2 and unconverted CO or H2; and recycling a first portion of the tail gas to the fermentor and/or a second portion of the tail gas to the acid-gas removal unit. This invention allows for increased syngas conversion to ethanol, improved process efficiency, and better overall biorefinery economics for conversion of biomass to ethanol.

Abstract: The disclosure provides genetically engineered C1-fixing microorganisms capable of producing nanobodies. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from nonessential or undesirable products towards products and/or co-products of interest. The disclosure enables co-production of useful chemicals from gaseous substrates.

Abstract: Disclosed herein are improved methods for production of 2-phenylethanol by microbial fermentation of substrates comprising carbon monoxide and/or carbon dioxide and further disclosed are genetically modified microorganisms for use in such methods that alleviate dependence on natural and petrochemical processes.

Abstract: Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from a species of Clostridia such as cellular extracts from Clostridium autoethanogenum.

Abstract: The disclosure provides methods for the production of liquefied petroleum gas from sustainable feedstocks, including methods comprising conversion of alcohols produced by gas fermentation for the production of propane and/or butane.

Abstract: Provided herein are microorganisms and methods for fermentative production of ?-ketoadipate from gaseous substrates such as carbon dioxide (CO2), carbon monoxide (CO), and/or hydrogen (H2). Additionally, the processes provided herein are methods for producing polymers containing ?-ketoadipate, that can potentially enable a circular economy by diverting waste, e.g., plastic waste.

Abstract: Improving overall carbon capture specially carbon sequestration in soil and improving overall production yield of bio-derived chemical products and fuels by integrating microbial fermentation into nature-based solutions and natural climate solutions. Converting feedstock of biomass and optionally supplemental bio-derived material, wherein the biomass comprises harvested biodiverse above-ground plant material comprising at least about 2, 3, 5, 8, 10, 12, 15 or 20 different species of plants from an area of land wherein the biodiverse above-ground plant material is harvested no more than one, two, or three times in a 12-month period of time to one or more products. In certain aspects, also disclosed are to processes for producing desirable products, such as ethylene, from biodiverse biomass.

Abstract: The systems and methods disclosed herein provide for the efficient generation of fine bubbles. In particular, systems and methods for use in bioreactors are disclosed herein providing a superior means to produce useful fermentation products by the biological fermentation of fine bubble waste substrates injected into a liquid broth containing a microorganism culture.

Abstract: The disclosure provides a system and a process for injecting and uniformly distributing a liquid into a fermentation broth in a bioreactor for maintaining stable condition within the fermentation broth. The system includes a downcomer section which contains two or more circular pipe conduits extending from a wall of the downcomer to the center vertical axis line of the downcomer. Liquid injection apertures are arranged along the circular pipe conduit and are non-uniformly spaced apart from the center vertical axis of the downcomer to the wall of the downcomer. The apertures further alternate and are offset between the top and bottom surfaces of the circular pipe conduit. The process of the disclosure involves using the system described above for carrying out a fermentation process for converting a C1-substrate to at least one product such as ethanol.

Abstract: The disclosure provides genetically engineered microorganisms and methods for improved biological production of ethylene glycol and precursors of ethylene glycol. The microorganism of the disclosure produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The disclosure further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Abstract: The disclosure provides for the integration of a gas fermentation process with a gasification process whereby tail gas from the gas fermentation process is recycled to a dryer of the gasification process. The tail gas from the gas fermentation process is utilized to generate heat which in turn is used to dry feedstock to the gasification process. The heat is typically used to heat a drying gas, such as air, which is then directly or indirectly contacted with the gasification feedstock to dry the gasification feedstock. Dried gasification feedstock provides improved yield and improved quality of syngas as compared to gasification feedstock that is not dried.

Abstract: Systems and methods relating to dynamic spargers for generating fine bubbles within reactors such as biological and chemical reactors. A sparger system is positioned within a reactor and comprises a support plate, multiple annular shrouds engaged with the support plate, and spargers positioned within the annular shrouds defining a gap between an interior surface of the annular shroud and an exterior surface of the corresponding sparger. Liquid flows through the defined gap between an interior surface of the annular shroud and an exterior surface of the sparger. Acceleration of the liquid through the gap shears bubbles at the exterior surface of the sparger creating bubbles or fine bubbles.