Patents Assigned to LaserGen, Inc.
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3?-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Patent number: 10041115Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: GrantFiled: May 23, 2017Date of Patent: August 7, 2018Assignee: LASERGEN, INC.Inventors: Brian P. Stupi, Hong Li, Weidong Wu, Megan N. Hersh, David Hertzog, Sidney E. Morris, Michael L. Metzker -
3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING
Publication number: 20170327886Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: ApplicationFiled: May 23, 2017Publication date: November 16, 2017Applicant: LASERGEN, INC.Inventors: Brian P. STUPI, Hong LI, Weidong WU, Megan N. HERSH, David HERTZOG, Sidney E. MORRIS, Michael L. METZKER -
3?-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Patent number: 9689035Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: GrantFiled: June 21, 2016Date of Patent: June 27, 2017Assignee: LASERGEN, INC.Inventors: Brian P. Stupi, Hong Li, Weidong Wu, Megan N. Hersh, David Hertzog, Sidney E. Morris, Michael L. Metzker -
Publication number: 20170052175Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.Type: ApplicationFiled: August 8, 2016Publication date: February 23, 2017Applicant: LASERGEN, INC.Inventors: Mimi HEALY, Xiyi CHEN, Jinchun WANG, Weidong WU, Sophia PETRICHENKO, Su ZHANG, Stacie FRYE
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3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING
Publication number: 20160369336Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: ApplicationFiled: June 21, 2016Publication date: December 22, 2016Applicant: LASERGEN, INC.Inventors: Brian P. STUPI, Hong LI, Weidong WU, Megan N. HERSH, David HERTZOG, Sidney E. MORRIS, Michael L. METZKER -
3?-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Patent number: 9399798Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: GrantFiled: October 1, 2014Date of Patent: July 26, 2016Assignee: LASERGEN, INC.Inventors: Brian P. Stupi, Hong Li, Weidong Wu, Megan N. Hersh, David Hertzog, Sidney E. Morris, Michael L. Metzker -
Patent number: 9200319Abstract: The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.Type: GrantFiled: October 21, 2014Date of Patent: December 1, 2015Assignee: Lasergen, Inc.Inventors: Vladislav A. Litosh, Megan N. Hersh, Brian P. Stupi, Weidong Wu, Michael L. Metzker
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3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING
Publication number: 20150176074Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: ApplicationFiled: October 1, 2014Publication date: June 25, 2015Applicant: LASERGEN, INC.Inventors: Brian P. STUPI, Hong LI, Weidong WU, Megan N. HERSH, David HERTZOG, Sidney E. MORRIS, Michael L. METZKER -
Publication number: 20150140559Abstract: The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.Type: ApplicationFiled: October 21, 2014Publication date: May 21, 2015Applicant: LASERGEN, INC.Inventors: Vladislav A. LITOSH, Megan N. HERSH, Brian P. STUPI, Weidong WU, Michael L. METZKER
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Patent number: 8969535Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.Type: GrantFiled: August 23, 2012Date of Patent: March 3, 2015Assignee: Lasergen, Inc.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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3?-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Patent number: 8889860Abstract: The present invention relates generally to 3?-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas: wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.Type: GrantFiled: September 13, 2012Date of Patent: November 18, 2014Assignee: Lasergen, Inc.Inventors: Brian Stupi, Hong Li, Weidong Wu, Megan N. Hersh, David Hertzog, Sidney E. Morris, Michael L. Metzker -
Patent number: 8877905Abstract: The present invention relates generally to labeled and unlabeled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.Type: GrantFiled: June 27, 2013Date of Patent: November 4, 2014Assignee: LaserGen, Inc.Inventors: Vladislav A. Litosh, Megan N. Hersh, Brian P. Stupi, Weidong Wu, Michael L. Metzker
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Patent number: 8497360Abstract: The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.Type: GrantFiled: February 28, 2012Date of Patent: July 30, 2013Assignee: LaserGen, Inc.Inventors: Vladislav A. Litosh, Megan N. Hersh, Brian P. Stupi, Weidong Wu, Michael L. Metzker
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Patent number: 8361727Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.Type: GrantFiled: May 24, 2011Date of Patent: January 29, 2013Assignee: Lasergen, Inc.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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Patent number: 8198029Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information.Type: GrantFiled: January 7, 2011Date of Patent: June 12, 2012Assignee: Lasergen, IncInventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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Patent number: 8148503Abstract: The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.Type: GrantFiled: June 11, 2009Date of Patent: April 3, 2012Assignee: Lasergen, Inc.Inventors: Vladislav A. Litosh, Megan N. Hersh, Brian P. Stupi, Weidong Wu, Michael L. Metzker
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Publication number: 20110287427Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.Type: ApplicationFiled: May 24, 2011Publication date: November 24, 2011Applicant: LASERGEN, INC.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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Patent number: 7964352Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.Type: GrantFiled: November 11, 2008Date of Patent: June 21, 2011Assignee: LaserGen, Inc.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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Patent number: 7897737Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.Type: GrantFiled: December 5, 2006Date of Patent: March 1, 2011Assignee: LaserGen, Inc.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
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Patent number: 7893227Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information.Type: GrantFiled: December 5, 2006Date of Patent: February 22, 2011Assignee: LaserGen, Inc.Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker