Patents Assigned to Le Centre National De Recherche Scientifique (CNRS)
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Publication number: 20160319306Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.Type: ApplicationFiled: June 24, 2016Publication date: November 3, 2016Applicants: Total Marketing Services, Le Centre National De Recherche Scientifique (CNRS), Université D'Aix Marseille, L'Institut National des Sciences Appliquées (INSA)Inventors: Laurence GIRBAL, Florence Saint-Prix, Mathieu Cesari, Henri-Pierre Fierobe, Roberto Mazzoli, Florence Mingardon, Angélique Chanal-Vial
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Patent number: 9441213Abstract: Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.Type: GrantFiled: April 9, 2014Date of Patent: September 13, 2016Assignees: Total Marketing Services, Le Centre National De Recherche Scientifique (CNRS), Université d'Aix-Marseille, L'Institut National des Sciences Appliquées (INSA)Inventors: Henri-Pierre Fierobe, Florence Mingardon, Angélique Chanal-Vial
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Patent number: 9382306Abstract: The present invention relates to novel octapeptide compounds of general formula (I): H-2-Nal 1-cyclo(Cys2-Tyr3-AA4-Lys5-Val6-Cys7)-Thr8-NH2. Since these products have a good affinity for certain somatostatin receptor subtypes, they are particularly advantageous for treating pathological states or diseases in which one (or more) somatostatin receptor(s) is (are) involved. These compounds furthermore have physiochemical properties that make it possible to envisage them in diverse solutions for the formulation of medicaments, for example as a pharmaceutically acceptable carrier. The invention also relates to pharmaceutical compositions containing said products and to the use thereof for the preparation of a medicament.Type: GrantFiled: September 29, 2009Date of Patent: July 5, 2016Assignees: IPSEN PHARMA S.A.S., COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA), LE CENTRE NATIONAL DE RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Marie-Thérèse Paternostre, Jean-Christophe Cintrat, Céline Valery, Stéphane Roux, Bernard Rousseau, Maarten Ijsselstijn, Roland Cherif-Cheikh, Franck Artzner
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Patent number: 8871479Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.Type: GrantFiled: November 26, 2009Date of Patent: October 28, 2014Assignees: Total Marketing Services, Le Centre National de Recherche Scientifiques (CNRS), L'Institut National des Sciences Appliquees (INSA), Universite d'Aix-MarseilleInventors: Henri-Pierre Fierobe, Angélique Chanal-Vial, Anne-Laure Molinier, Chantal Tardif, Luc Dedieu
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Publication number: 20130115669Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.Type: ApplicationFiled: January 9, 2013Publication date: May 9, 2013Applicants: Total S.A., Le Centre National De Recherche Scientifique (CNRS, L'Institut National des Sciences Appliquées (INSA), L'Université de Provence, L'Université de la MediterranéeInventors: Laurence GIRBAL, Florence Saint-Prix, Mathieu Cesari, Henri-Pierre Fierobe, Roberto Mazzoli, Florence Mingardon, Angélique Chanal-Vial
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Publication number: 20120122176Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.Type: ApplicationFiled: November 26, 2009Publication date: May 17, 2012Applicants: Total S.A., Le Centre National De Recherche Scientifique (CNRS), L'Institut National des Sciences Appliquees (INSA), L'Universite de Provence, L'Universite de la MediterraneeInventors: Henri-Pierre Fierobe, Angélique chanal-Vial, Anne-Laure Molinier, Chantal Tardif, Luc Dedieu
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Publication number: 20110294184Abstract: The invention relates to applications of a cellulase of Pseudomonas sp. ND137, functional fragments and/or variants and engineered forms thereof, in the context of industrial bioprocessing, more particularly solvent production.Type: ApplicationFiled: November 26, 2009Publication date: December 1, 2011Applicants: Total S.A., Le Centre National De Recherche Scientifique (CNRS), L'Institut National des Sciences Appliquees (INSA), L'Universite de Provence, L'Universite de la MediterraneeInventors: Henri-Pierre Fierobe, Angélique Chanal-Vial
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Publication number: 20110236943Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.Type: ApplicationFiled: December 2, 2009Publication date: September 29, 2011Applicants: Total S.A, Le Centre National de Recherche Scientifique (CNRS), L'Universite de la Mediterranee, L'Universite de Provence, L'Institut National des Sciences Appliquees (INSA)Inventors: Laurence Girbal, Florence Saint-Prix, Mathieu Cesari, Henri-Pierre Fierobe, Roberto Mazzoli, Florence Mingardon, Angélique Chanal-Vial
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Publication number: 20110178013Abstract: The present invention relates to novel octapeptide compounds of general formula (I): H-2-Nal1-cyclo(Cys2-Tyr3-AA4-Lys5-Val6-Cys7)-Thr8-NH2. Since these products have a good affinity for certain somatostatin receptor subtypes, they are particularly advantageous for treating pathological states or diseases in which one (or more) somatostatin receptor(s) is (are) involved. These compounds furthermore have physiochemical properties that make it possible to envisage them in diverse solutions for the formulation of medicaments, for example as a pharmaceutically acceptable carrier. The invention also relates to pharmaceutical compositions containing said products and to the use thereof for the preparation of a medicament.Type: ApplicationFiled: September 29, 2009Publication date: July 21, 2011Applicants: Ipsen Pharma S.A.S., Commissariat a L'Energie Atomique Aux Energies Alternatives (C.E.A.), Le Centre National De Recherche Scientifique (CNRS)Inventors: Marie-Thérèse Paternostre, Jean-Christophe Cintrat, Céline Valery, Stéphane Roux, Bernard Rousseau, Maarten Ijsselstijn, Roland Cherif-Cheikh, Frank Artzner
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Publication number: 20110129876Abstract: Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.Type: ApplicationFiled: July 30, 2009Publication date: June 2, 2011Applicants: Total S.A., Le Centre National De Recherche Scientifique (CNRS), L'Universite de la Mediterranee, L'Universite de Provence, L'Instutut National des Sciences Appliquees (INSA)Inventors: Henri-Pierre Fierobe, Florence Mingardon, Angélique Chanal-Vial