Abstract: A specimen box for an electron microscope capable of observing a general specimen or a live cell is formed of a housing. The housing includes a receiving chamber formed therein and at least one view hole formed on each of a top side thereof and a bottom side thereof and communicating with the receiving chamber and coaxially aligned with the other. The distance between a bottom end of the view hole located on the top side of the housing and a top end of the view hole located on the bottom side of the housing is smaller than 50 ?m. The housing is ultra-thin to enable penetration of the electron beam therethrough even though a liquid specimen is injected into the housing. A general specimen or a live cell can be put into the housing for the microscopic observation under the electron microscope.

Abstract: A closed observational device for an electron microscope is formed of a housing. The housing includes a liquid chamber formed therein, at least one view hole formed at each of a top side thereof and a bottom side thereof and communicating with the liquid chamber and coaxially aligned with the other, and a film mounted to and sealing each of the view holes. Accordingly, a general specimen or a live cell can be placed into the liquid chamber for microscopic observation under the electron microscope. Besides, the present invention can enclose the liquid inside the housing to prevent the liquid from exhausting outward or volatilization.

Abstract: An ultra-thin liquid control plate and a combination of a box-like member and the control plat include a plate-like member having at least one view hole. The joint surface combined with a sidewall of the view hole and a surface of the plate-like member is provided with at least one more-hydrophilic section and at least one less-hydrophilic section, wherein the more-hydrophilic section has a height smaller than 50 ?m. While combined with the box-like member, the plate-like member is mounted in the box-like member. The box-like member has at least one through hole running through each of a top side and a bottom side thereof and coaxially aligned with the view hole. After a liquid is placed into the view hole, the more-hydrophilic section adsorbs the liquid to form a liquid layer and the less-hydrophilic section is hydrophobic to adsorb no liquid.

Abstract: A method of observing a live unit under an electron microscope includes the steps of (A) preparing a live environment inside the electron microscope, wherein the live environment is provided with at least one live unit and a predetermined environmental condition keeping basic physiology of the live unit functional, at least one pair of view windows is located opposite to each other, and the live unit includes at least two objects that can bear different critical charge densities respectively; and (B) irradiating the live unit with a particle beam of predetermined intensity through the view windows, and then display the live unit on an imaging device of the electron microscope, wherein the product of the predetermined intensity of the particle beam and the predetermined duration equals the predetermined charge density that is smaller than or equal to the critical charge density of the object of the live unit.

Abstract: A method of operating liquid in a vacuum or low-pressure environment and observing the operation and a device for the operation and the observation respectively, including the steps of preparing a housing, putting the housing in the vacuum or low-pressure environment and keep a predetermined temperature difference between liquid, vapor, and buffer chambers, infusing vapor into the vapor chamber through a gas inlet and control the vapor pressure inside the vapor chamber to be equal to the saturated vapor pressure of a liquid specimen inside the liquid chamber under the same temperature to prevent the inside liquid from volatilization, and evacuating the buffer chamber through the pumping port to pump out the vapor and prevent the vapor from exhausting through outer apertures out of the housing. A probing source can pass through the outer, inner, and vapor apertures for observation and analysis of the liquid specimen inside the liquid chamber.

Abstract: A nanoimprint lithography method of fabricating a nanoadhesive includes steps of (a) preparing a substrate and a mold under the vacuum environment, wherein at least one of the substrate and the mold is transparent, the mold is located over the substrate and has an oppressing portion having nanometer-scale features and a mold release agent located on the surface of the nanometer-scale features; (b) coating a liquid resist cast on the substrate, wherein the resist cast can be hardened by ultraviolet rays; (c) having the mold is pressed on the substrate to enable the resist cast to fill between the nanometer-scale features and the substrate; (d) irradiating the resist cast by the ultraviolet rays for hardening; and (e) releasing the mold from the substrate to enable the resist cast to produce a contrast pattern thereon corresponding to the nanometer-scale features, wherein the resist cast with the contrast pattern is the nanoadhesive.