Abstract: A scanning microscope has at least one illumination source for emitting a light beam, which is fed via a microscope optic to a specimen and scans the latter. In order to correct the imaging defect of the microscope optic, said defect is determined and a correction value is determined therefrom. This correction value is used for influencing control signals which control the impinging of the light beam on the specimen.

Abstract: An arrangement is proposed for detecting fluorescent light from a plurality of specimen points (2), in particular from microgene spots or microbiospots, the specimen points (2) being arranged on a slide (1). This arrangement comprises at least one light source for simultaneously illuminating the specimen points (2) with excitation light, and detection means, comprising detection elements (3), for simultaneously detecting the fluorescent light from the individual specimen points (2). According to the invention, the spacing d between the specimen points (2) and the respectively assigned detection elements (3) is selected to be as small as possible.

Abstract: An optical arrangement in the beam path of a light source suitable for fluorescence excitation, preferably in the beam path of a confocal laser scanning microscope, with at least one spectrally selective element (4) to inject the excitation light (3) of at least one light source (2) in the microscope and to extract the excitation light (3) scattered and reflected on the object (10) or the excitation wavelength from the light (13) coming from the object (10) through the detection beam path (12) is characterized for variable configurations with the simplest construction in that excitation light (3, 9) of different wavelengths can be extracted by the spectrally selective element (4). Alternatively, an optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be extracted.

Abstract: A microscope with a light source that emits light for illumination of a specimen and with a spectrometer that receives detection light proceeding from the specimen, has an optical arrangement which has an acoustooptical component, and which directs the light of the light source to the specimen and delivers the detection light proceeding from the specimen to a spectrometer in spectrally undivided fashion. A flow cytometer and a method for examination of a specimen are also disclosed.

Abstract: A method and an arrangement for beam control in a scanning microscope are disclosed. The scanning microscope comprises means for acquiring and displaying (3) a preview image (7) and a microscope optical system (51). Means for marking (5) at least one region of interest (27, 29) in the preview image (7) are provided. A first beam deflection device (43, 67, 68) displaces the scan field (31, 33) onto the region of interest (27, 29); and a second beam deflection device (49, 72, 94) serves for meander-shaped scanning within the scan field (31, 33).

Abstract: The invention concerns an arrangement for scanning a specimen receiving device (1) for data recording with a laser scanning microscope, preferably with a confocal laser scanning microscope (2), with which imaging of large specimen fields with sufficient speed is possible by scanning of the specimen receiving device (1), which is characterized in that the specimen receiving device (1) is alternatingly rotatable about a first axis (4) by a rotation device (3).

Abstract: A microscope objective that comprises an objective housing and contains several lens elements, at least one lens element being arranged displaceably in motor-driven fashion within the objective housing, is disclosed. A microscope and a method for imaging a specimen are additionally disclosed.

Abstract: The present invention relates to an optical arrangement for illuminating objects (1), in particular fluorescent objects, preferably in conjunction with a confocal or a double-confocal scanning microscope, having an illuminating beam path (2) of a light source (3), a detection beam path (4) of a detector (5), and a component (6) which unifies the detection beam path (4). For the purpose of at least largely loss-free union of the light coming from the object (1) into a propagation direction (19), the optical arrangement according to the invention is characterized in that with reference to the beam cross section active for the detector, light of the fist and second partial detection beam can be united at least largely in an overlapping fashion into one propagation direction (19) at the component (6) thereby providing an unified the detection beam path (4).

Abstract: A scanning microscope with a light source for illumination of a specimen, a means for spatial spectral division of the detection light, and a detector is disclosed. The scanning microscope has means for selecting a lower limit wavelength that defines a lower exclusion region, and means for selecting an upper limit wavelength that defines an upper exclusion region, as well as a first and a second adjustable stop that block light components of the lower and the upper exclusion region of the detection light. A bandpass filter and a method for scanning microscopy are also disclosed.

Abstract: A method for generating a multicolor image of a specimen with a microscope is disclosed. The method comprises the step of determining the spacing of the focal planes of a first illuminating light beam that has a first wavelength and of a second illuminating light beam that has a second wavelength; the step of scanning the specimen with the first illuminating light beam and generating a first partial image; the step of performing a relative displacement, by an amount equal to the spacing, between the specimen and the focal plane of the illuminating light beam of the second wavelength; the step of scanning the specimen with the second illuminating light beam and generating a second partial image; and the step of superimposing the first and second partial images to yield the multicolor image. Further more a microscope and a confocal scanning microscope are disclosed.

Abstract: A Vibration damping device for a microscope comprises a housing. In the housing a foam rubber defining a cavity is formed which encloses a weight. The foam rubber has a plurality of interconnected pores. The housing is of the vibration damping device has in one embodiment a rectangular shape.

Abstract: The invention discloses an entangled-photon microscope (1) having a light source (3) and an objective (31). The entangled-photon microscope (1) has a microstructured optical element (11), in which entangled photons can be produced, between the light source (3) and the objective (31), the entangled photons propagating in a beam (15) inside and outside the microstructured optical element.

Abstract: A method for operating a positioning apparatus that moves a positioning element comprises the steps of: generating a first and at least one further reference signal, the first reference signal corresponding to a first reference position and the further reference signal to a further reference position; generating a first and at least one further estimated value for the present position of the positioning drive; transferring the first reference signal and the estimated value to the positioning apparatus; establishing the first reference position using the positioning apparatus, and measuring the present position and generating a position signal that corresponds to the measured present position; transferring the further reference signal to the positioning apparatus; generating a further estimated value of the positioning drive position; transferring the further estimated value to the positioning apparatus; establishing the further reference position, and measuring a further present position and generating a furt

Abstract: An apparatus for illuminating a specimen (1), preferably in confocal fluorescence scanning microscopy is disclosed. Two illumination beam paths (2, 4) are combined with a beam splitter (6) in order to simplify alignment and reduce the optical components in the illumination beam path. The at least one optical component (7) is arranged at least in one of the illumination beam paths (2, 4) and modifies the light; and that the optical properties of the component (7) can be influenced or modified in such a way that the illumination pattern of the illumination beam path (2, 4) in the specimen region changes shape.

Abstract: A method and an apparatus for illuminating a transparent specimen (1), in particular for use in double confocal scanning microscopy, wherein for illumination of a point of the specimen (1), two light waves of a coherent light source (4) focused from opposite directions (2, 3) onto the point interfere to form an illumination pattern, and in order to eliminate the causes of the problems of the reconstruction method, at least two additional coherent light waves traveling toward one another are superimposed in order to minimize the secondary maxima (11, 12) of the illumination pattern.

Abstract: The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.

Abstract: A method and an apparatus for measuring thickness of transparent films is described in which an illumination beam is directed through an objective onto an object comprising a transparent film. A structured focusing aid is disposed in the illumination beam, a camera is disposed in an imaging beam. The focusing aid and the camera each are disposed in locations conjugated with the focal plane of the objective. The focal plane of the objective is displaced stepwise through the object. At each position, a camera image is recorded and its focus score is determined, the image of the focusing aid being used as the contrast indicator. The positions with maximal focus scores are assigned to the locations of the interfaces. The thickness of the transparent film is calculated from the difference between the positions of its interfaces.

Abstract: A scanning microscope is disclosed. The scanning microscope comprises a light source which emits an illuminating light beam for illumination of a specimen, a resonant beam deflection device, for guiding the illuminating light beam over the specimen, which has a resonant frequency and a resonant frequency range, and an independent oscillator with which a drive oscillation, which has a drive frequency within the resonant frequency range can be generated which drives the beam deflection device. Furthermore a method is disclosed for controlling a scanning microscope having a resonant beam deflection.

Abstract: A microscope system (4) for the observation of dynamic processes comprises a microscope (50) having at least one detector (19), and a computer (34). A buffer memory (54) precedes a comparator (58) that compares image contents of at least two successive image frames (56k and 56k+1). Depending on the result obtained from the comparator (58), the image frames are stored in different segments of a data structure (66) that is provided.

Abstract: A method of finding, recording and optionally evaluating object structures, especially on slides, preferably of fluorescent object structures such as gene spots. A microscope with a CCD camera, a scanning microscope or a preferably confocal laser scanning microscope can be used for recording and for the rapid and reliable detection of the object structures, with the image data being recorded using an illumination pattern that is projected into the object plane.