Abstract: Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.
Type:
Grant
Filed:
June 8, 2017
Date of Patent:
November 26, 2019
Assignees:
Lekarska fakulta v Plzni, Univerzita Karlova (Faculty of Medicine in Pilsen, Charles University), The Curators of the University of Missouri, Chonbuk National University, Division of Biotechnology, College of Environmental and Bioresources, Ceska zemedelska univerzita v Praze, Institute of Animal Science
Inventors:
Jan Nevoral, Young-Joo Yi, Katerina Zamostna-Adamkova, Jaroslav Petr, Peter Sutovsky, Milena Kralickova