Patents Assigned to LEXOGEN GMBH
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Patent number: 11410747Abstract: The invention relates to a method of estimating a nucleic acid copy number after amplification comprising the steps of providing a sample comprising nucleic acids of a to be determined number of copies; attaching variable labels to said nucleic acids; amplifying said nucleic acids with the labels with a nucleic acid duplication procedure, preferably PCR; determining amounts of amplified nucleic acid copies with a label, each amount is determined for nucleic acid copies with a different label; and providing an estimation of the nucleic acid copy number in the sample based on the determined amounts of amplified nucleic acid copies; as well as computer program products adapted for performing such methods.Type: GrantFiled: September 21, 2018Date of Patent: August 9, 2022Assignee: LEXOGEN GMBHInventors: Andreas Turk, Michael Moldaschl
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Patent number: 11021705Abstract: A method of ligating DNA molecules, wherein the DNA molecules are in a hybrid with an RNA molecule, including the steps of providing DNA molecules that are in a RNA:DNA hybrid with an RNA molecule, and ligating the DNA molecules to each other with a double strand specific ligase.Type: GrantFiled: August 10, 2017Date of Patent: June 1, 2021Assignee: LEXOGEN GMBHInventors: Alexander Seitz, Pamela Moll, Magdalena Anna Napora
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Patent number: 10612018Abstract: The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3? end of said oligonucleotide stopper—said stopper may also be a primer itself and uses and kits for performing said method.Type: GrantFiled: September 17, 2012Date of Patent: April 7, 2020Assignee: LEXOGEN GMBHInventors: Alexander Seitz, Pamela Moll, Magdalena Anna Napora
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Patent number: 10597699Abstract: The present invention provides a method for generating an amplified nucleic acid portion of a template RNA molecule, comprising after having obtained a template RNA, annealing a first oligonucleotide primer at a preselected 3? terminal nucleic acid region of the template RNA, elongating the first oligonucleotide primer in a template specific manner thereby obtaining a first elongated strand, removing the RNA template, annealing one or more further oligonucleotide primers to the first elongated strand, elongating the one or more further oligonucleotide primers in a template specific manner without strand displacement of polynucleotides annealed to the first elongated strand or using a polymerase that destroys a displaced strand, thereby generating further elongation products, isolating and/or amplifying an elongation product of said further elongation product comprising a nucleic acid portion that is elongated complementary to the first oligonucleotide primer; as well as kits for performing the method.Type: GrantFiled: March 20, 2015Date of Patent: March 24, 2020Assignee: LEXOGEN GMBHInventor: Pamela Moll
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Patent number: 10538795Abstract: The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5? protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5? protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and optionally modifying the extension product of said nucleic acids without 5? protecting group either on the 5? end of the template strand or on the 3? end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified, wherein therefore the labelled nucleic acid dType: GrantFiled: July 10, 2013Date of Patent: January 21, 2020Assignee: LEXOGEN GMBHInventors: Alexander Seitz, Irmlind Gabler, Lukas Paul
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Patent number: 10513726Abstract: The present invention relates to the field of transcriptomics and provides a method for the controlled identification and/or quantification of transcript variants in samples, comprising providing a reference set of artificial polynucleic acid molecules simulating transcript variants and adding said reference set as external control to samples comprising transcript variants. The present invention further provides such a reference set, as well as a method to produce such a reference set.Type: GrantFiled: July 9, 2015Date of Patent: December 24, 2019Assignee: LEXOGEN GMBHInventors: Lukas Paul, Petra Kubala, Torsten Reda
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Patent number: 9862996Abstract: The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.Type: GrantFiled: February 27, 2012Date of Patent: January 9, 2018Assignee: Lexogen GmbHInventors: Torsten Reda, Jakob Haglmueller, Georg Schitter, Alexander Seitz
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Publication number: 20170321248Abstract: The present invention relates to the field of transcriptomics and provides a method for the controlled identification and/or quantification of transcript variants in samples, comprising providing a reference set of artificial polynucleic acid molecules simulating transcript variants and adding said reference set as external control to samples comprising transcript variants. The present invention further provides such a reference set, as well as a method to produce such a reference set.Type: ApplicationFiled: July 9, 2015Publication date: November 9, 2017Applicant: LEXOGEN GMBHInventors: Lukas Paul, Petra Kubala, Torsten Reda
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Patent number: 9540683Abstract: We provide a sensor array device for the measurement of mixtures of organic compounds comprising an assembly of sensor half elements which have been functionalized through sensor compounds before the assembly. Each individual sensor of the array contains two sensor compounds which are bound at opposite sensor half elements. The molecular recognition is bi-functional. While the amount of sensor compounds increases linearly, the individual sensors increase with the second power.Type: GrantFiled: February 27, 2012Date of Patent: January 10, 2017Assignee: Lexogen GmbHInventors: Torsten Reda, Igor Holländer, Alexander Seitz
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Patent number: 9394564Abstract: The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3? end of the transcribed cDNA with a polynucleotide tail after the 3? end, c) amplification of the cDNA using a pair of primers, wherein a first 3? primer is specific for the 5? end of the cDNA and a second 5? primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3?polynucleotide tail of the cDNA.Type: GrantFiled: November 29, 2006Date of Patent: July 19, 2016Assignee: LEXOGEN GMBHInventor: Alexander Seitz
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Patent number: 9334532Abstract: A method for the reduction of the complexity of nucleic acid pool(s), comprising: providing a sample with one or more nucleic acid molecules; cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments; and amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, wherein the amplified or amplifying one or more fragments of said fraction are divided into different subpools, and wherein the fragments of each subpool comprise a common nucleic acid feature.Type: GrantFiled: February 2, 2011Date of Patent: May 10, 2016Assignee: LEXOGEN GMBHInventors: Alexander Seitz, Jakob Haglmüller, Torsten Reda
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Publication number: 20150184235Abstract: A sensor carrier contains a flexible film supporting a plurality of functionalized sensor elements each being formed by a functional layer. The functional layers are located on the same surface of the film within a window area, A region of each of the functional layers of the sensor elements is functionalized, and one or more sensor compounds are arranged or located in the respective functionalized regions of the sensor elements.Type: ApplicationFiled: July 10, 2012Publication date: July 2, 2015Applicant: LEXOGEN GMBHInventors: Thorsten Reda, Igor Hollaender
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Publication number: 20140018262Abstract: We provide a sensor array device for the measurement of mixtures of organic compounds comprising an assembly of sensor half elements which have been functionalized through sensor compounds before the assembly. Each individual sensor of the array contains two sensor compounds pounds which are bound at opposite sensor half elements. The molecular recognition is bi-functional. While the amount of sensor compounds increases linearly, the individual sensors increase with the second power.Type: ApplicationFiled: February 27, 2012Publication date: January 16, 2014Applicant: LEXOGEN GMBHInventors: Torsten Reda, Igor Holländer, Alexander Seitz
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Publication number: 20130331299Abstract: The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.Type: ApplicationFiled: February 27, 2012Publication date: December 12, 2013Applicant: LEXOGEN GMBHInventors: Torsten Reda, Jakob Haglmueller, Georg Schitter, Alexander Seitz
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Publication number: 20120289412Abstract: The present invention relates to a method for the reduction of the complexity of nucleic acid pool(s), comprising providing a sample with one or more nucleic acid molecules, cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, and wherein the amplified or amplifying one or more fragments of said fraction are divided into different sub-pools, and wherein the fragments of each subpool comprise a common nucleic acid feature.Type: ApplicationFiled: February 2, 2011Publication date: November 15, 2012Applicant: LEXOGEN GMBHInventors: Alexander Seitz, Jakob Haglmüller, Torsten Reda
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Publication number: 20120238457Abstract: The present invention relates to a method of ordering nucleic acid molecule fragment sequences derived from a pool of potentially diverse RNA molecules comprising optionally reverse transcribing the RNA molecules to provide a pool of cDNA molecules, segregating nucleic acids from said template RNA or cDNA pool, selecting for potentially different templates with a distinctive nucleic acid feature shared by the segregated templates, thereby providing at least a first subpool of nucleic acids, optionally once or more further segregating nucleic acids from said template RNA or cDNA, selectively segregating nucleic acids with a different distinctive nucleic acid feature, thereby providing one or more further subpool(s) of nucleic acids, generating fragments of said segregated nucleic acid molecules by fragmenting or obtaining fragment copies of said segregated nucleic acid molecules, wherein the fragments of each subpool or combined subpools remain separable from fragments of other subpools or other combined suType: ApplicationFiled: December 10, 2010Publication date: September 20, 2012Applicant: LEXOGEN GMBHInventors: Alexander Seitz, Lukas Paul, Max Jan Van Min
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Publication number: 20090311754Abstract: The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3? end of the transcribed cDNA with a polynucleotide tail after the 3? end, c) amplification of the cDNA using a pair of primers, wherein a first 3? primer is specific for the 5? end of the cDNA and a second 5? primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3?polynucleotide tail of the cDNA.Type: ApplicationFiled: November 29, 2006Publication date: December 17, 2009Applicant: LEXOGEN GMBHInventor: Alexander Seitz