Abstract: This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.
Type:
Grant
Filed:
August 31, 2005
Date of Patent:
January 19, 2010
Assignee:
Life Technologies, Corp.
Inventors:
Joel A. Jessee, Fredric R. Bloom, Thuan Trinh