• Publication number: 20110033845
    Abstract: The teachings relate to methods and kits for detecting whether target nucleic acid sequences are present and/or quantitating target nucleic acid sequences.
    Type: Application
    Filed: January 9, 2009
    Publication date: February 10, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventor: Eugene G. SPIER
  • Publication number: 20100323406
    Abstract: The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
    Type: Application
    Filed: August 17, 2009
    Publication date: December 23, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Paolo Vatta, John W. Brandis, Elena V. Bolchakova, Sandra L. Spurgeon
  • Publication number: 20100267118
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: July 2, 2009
    Publication date: October 21, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
  • Publication number: 20100261231
    Abstract: Novel cap analogs which are easily synthesized, resulting in high levels of capping efficiency and transcription and improved translation efficiencies are provided. Such caps are methylated at the N7 position of one or both guanosines of the dinucleotide cap as well as at the 3? position on the ribose ring. Substituent groups on the ribose ring also result in the cap being incorporated in the forward orientation. Also provided are methods useful for preparing capped analogs and using mRNA species containing such analogs are also contemplated herein, as well as kits containing the novel cap analogs.
    Type: Application
    Filed: July 10, 2007
    Publication date: October 14, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Anilkumar R. Kore, Shanmugasundaram Muthian
  • Publication number: 20100029925
    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
    Type: Application
    Filed: May 22, 2009
    Publication date: February 4, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Maxim G. Brevnov, Jaiprakash Shewale, Manohar R. Furtado, Hemant S. Pawar
  • Publication number: 20100015621
    Abstract: The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.
    Type: Application
    Filed: June 30, 2009
    Publication date: January 21, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Chien-Wei Chang, Lori K. Hennessy, Dennis Y. Wang
  • Publication number: 20090308736
    Abstract: Apparatus and methods for separating different polynucleotide populations in a mixture are provided, wherein different polynucleotides or polynucleotide populations are captured on different solid support. After hybridization, polynucleotides are selectively released from a selected support by altering a physical property of that support. The released polynucleotides can be eluted from a common flow path and isolated.
    Type: Application
    Filed: March 9, 2009
    Publication date: December 17, 2009
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Janice G. Shigeura, Jer-Kang Chen, John Shigeura
  • Publication number: 20090215062
    Abstract: A composition for sequencing DNA is provided and comprises a nuclease and a nuclease-resistant sequencing primer. A method of preparing DNA for sequencing and a method of sequencing DNA are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the nuclease-resistant sequencing primer is essentially non-degraded.
    Type: Application
    Filed: February 3, 2009
    Publication date: August 27, 2009
    Inventor: LINDA G. LEE
  • Publication number: 20090131662
    Abstract: This invention is related to compositions and methods pertaining to PNA synthons, PNA oligomers and/or PNA/DNA Chimeras comprising a universal base.
    Type: Application
    Filed: January 23, 2009
    Publication date: May 21, 2009
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Birenda K. Bhattacharya, James M. Coull