Patents Assigned to Life Technologies
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Patent number: 12168800Abstract: Methods for evaluating microsatellite instability (MSI) analyze nucleic acid sequence reads corresponding to a plurality of marker regions for MSI. The marker regions may include long homopolymers and/or short tandem repeats (STRs). For a target homopolymer, a histogram of homopolymer signal values is calculated based on flow space signal measurements for the homopolymer region in the sequence reads. A score per marker based on features of the histogram of homopolymer signal values is determined for each marker region corresponding to the target homopolymers. For a target STR, the method includes calculating a histogram of repeat lengths for sequence reads corresponding to the marker region of the target STR. A score per STR marker is calculated based on features of the histogram of repeat lengths. A plurality of per marker scores may be combined to form a total MSI score for the sample.Type: GrantFiled: December 5, 2023Date of Patent: December 17, 2024Assignee: Life Technologies CorporationInventor: Sameh El-Difrawy
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Patent number: 12163188Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.Type: GrantFiled: September 26, 2022Date of Patent: December 10, 2024Assignee: Life Technologies CorporationInventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
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Patent number: 12152235Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.Type: GrantFiled: December 20, 2022Date of Patent: November 26, 2024Assignee: Life Technologies ASInventor: Laure Jobert
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Patent number: 12153014Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.Type: GrantFiled: February 10, 2023Date of Patent: November 26, 2024Assignees: Integenx, Inc., Life Technologies CorporationInventors: Jen-Kuei Liu, Bharti Solanki, Hans Michael Wenz, Frank Mercer, Cevat Akin, Achim Karger, Annelise Barron, Scott Mack
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Patent number: 12145926Abstract: The present application relates to a compound of Formula (I) or a pharmaceutically acceptable salt and/or solvate thereof, or a radionuclide complex comprising a compound of Formula (I) or a pharmaceutically acceptable salt and/or solvate thereof, and one or more radionuclides, and to compositions thereof. The present application also includes methods of using the compounds, complexes and compositions for targeting neurotensin receptors, and further to their use in the imaging, diagnosis and treatment of diseases, disorders or conditions such as cancer. The present application relates to a compound of Formula (II) or a pharmaceutically acceptable salt and/or solvate thereof, and to compositions and uses thereof, for example, in the treatment of diseases, disorders or conditions such as cancer.Type: GrantFiled: March 13, 2024Date of Patent: November 19, 2024Assignee: Full-Life Technologies HK LimitedInventor: Fa Liu
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Patent number: 12146853Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.Type: GrantFiled: December 11, 2023Date of Patent: November 19, 2024Assignee: Life Technologies CorporationInventors: James Bustillo, Mark J. Milgrew, Wolfgang Hinz, John Leamon, John Davidson, Martin Huber, Antoine M. van Oijen, Jonathan Rothberg
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Patent number: 12140544Abstract: A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.Type: GrantFiled: April 27, 2023Date of Patent: November 12, 2024Assignees: Life Technologies Holdings PTE LTD, Life Technologies CorporationInventors: Ming Song Chen, Chee Woei Chong, Wuh Ken Loh, Wern Yuh Foo, Kuan Moon Boo, Norbert Leclerc, Kristina Uhlendorf, Reiner Koellner, Torsten Fuchs, Stefan Breitfelder
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Patent number: 12140560Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: August 16, 2022Date of Patent: November 12, 2024Assignee: Life Technologies CorporationInventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
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Patent number: 12140559Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.Type: GrantFiled: December 22, 2022Date of Patent: November 12, 2024Assignee: Life Technologies CorporationInventors: Wolfgang Hinz, John Donohue, Daniel Beacham
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Patent number: 12139758Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.Type: GrantFiled: January 8, 2024Date of Patent: November 12, 2024Assignee: Life Technologies CorporationInventors: Philip Waggoner, James A. Ball, Michael L. Minto, David M. Cox, Wolfgang Hinz, Alexander Mastroianni, Jeremy Gray, Marc Glazer, Scott Parker, Kimberly Gorrell
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Patent number: 12139753Abstract: A method for detecting a gene fusion includes amplifying a nucleic acid sample in the presence of primer pool to produce a plurality of amplicons. The primer pool includes primers targeting a plurality of exon-exon junctions of a driver gene. The amplicons correspond to the exon-exon junctions. The amplicons are sequenced and aligned to a reference sequence. The number of reads corresponding to each amplicon is normalized to give a normalized read count. A baseline correction is applied to the normalized read counts for the amplicons to form corrected read counts. A binary segmentation score is calculated for each corrected read count. A predicted breakpoint for the gene fusion is determined based on the amplicon index corresponding to the maximum absolute binary segmentation score. Gene fusion events may be detected in a partner agnostic manner, i.e. without prior knowledge of the specific fusion partner genes or specific breakpoint information.Type: GrantFiled: March 20, 2020Date of Patent: November 12, 2024Assignee: Life Technologies CorporationInventors: Rajesh Gottimukkala, Amir Marcovitz, Jeoffrey Schageman, Varun Bagai, Jian Gu, James Veitch, Kelli Bramlett, Scott Myrand, Fiona Hyland, Seth Sadis, Paul Williams
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Patent number: 12122859Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.Type: GrantFiled: January 26, 2023Date of Patent: October 22, 2024Assignees: Life Technologies AS, Life Technologies CorporationInventors: Geir Fonnum, Grete Irene Modahl, Nini Hofsloekken Kjus, Astrid Evenroed Molteberg, Diem Thuy Thi Tran, Jo Aaserud, M. Talha Gokmen, Steven M. Menchen, Carl Fuller, Luisa Andruzzi, Wolfgang Hinz
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Patent number: 12110561Abstract: A DNA methylation profile of a few of trophectoderm cells in a blastocyst is detected, and the average methylation 5 level and the methylation pattern of embryos identified as having a good morphology in Gardner morphological blastocyst grading process are used as the standard for screening for well-developed blastocysts. The nearer the methylation level of the trophectoderm cells of the blastocyst to be tested approaches the methylation level or state of the good embryo, the better the embryo development is and the higher the 10 suitability for being implanted into a maternal subject is.Type: GrantFiled: April 11, 2017Date of Patent: October 8, 2024Assignee: Guangzhou NVWA Life Technology Co., Ltd.Inventors: Jiang Liu, Jie Qiao, Guoqiang Li, Yang Yu, Yong Fan, Congru Li
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Patent number: 12110995Abstract: A tube clamp for bioprocessing, comprising: a first clamp member (102) having a first end joined to a second end by a crescent shaped body comprising: an interior (118) having a groove (116) extending along the crescent shaped body and including a lower surface joined to a sidewall and a top surface joined to the sidewall by a tapered surface (124), wherein the tapered surface is angled at forty-five degrees relative to the lower surface and the top surface; an exterior surrounding the interior, the exterior including an exterior surface joined to an exterior sidewall by an exterior taper, the exterior taper is angled at forty-five degrees relative to the exterior surface and the exterior sidewall and the exterior sidewall is joined to the top surface; a closure region (112) joined to the first end; and an attachment region (110) joined to the second end; and a second clamp member having a first end joined to a second end by a crescent shaped body comprising: an interior including a groove extending along theType: GrantFiled: August 25, 2020Date of Patent: October 8, 2024Assignee: Life Technologies CorporationInventor: Gary Jenney
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Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
Patent number: 12098424Abstract: A method for designing test or control sequences may include identifying, using a variant caller, loci with systematic errors present in a plurality of sequencing runs included in a training set of sequencing runs obtained using sequencing-by-synthesis; and selecting a representative set of loci, including selecting from the identified loci an approximately equal number of loci involving errors in A, T, C, and G homopolymers and selecting from the identified loci an approximately equal number of loci involving homopolymers having a length of two, three, and four.Type: GrantFiled: August 20, 2020Date of Patent: September 24, 2024Assignee: Life Technologies CorporationInventor: Marcin Sikora -
Patent number: 12077818Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.Type: GrantFiled: April 7, 2023Date of Patent: September 3, 2024Assignee: Life Technologies CorporationInventors: Earl Hubbell, Christian Koller, Nils Homer
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Patent number: 12076242Abstract: An embodiment includes a chordal replacement system comprising: a pledget coupled to a first suture length and at least one of a first needle and a first ferrule; a second suture length coupled to the pledget and at least a second needle; and a third suture length coupled to the pledget and at least a third needle; wherein the second and third suture lengths are not monolithic with each other and do not constitute a single suture. Other embodiments are described herein.Type: GrantFiled: April 20, 2021Date of Patent: September 3, 2024Assignee: On-X Life Technologies, Inc.Inventors: Ryan Medema, Jeffrey Nelson, Anthony Lioi, Clyde Baker, Fletcher D. Southard, Mark E. Seeley
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Patent number: 12077742Abstract: A method for mixing a biological suspension includes disposing a biological suspension within a compartment of a container, the biological suspension comprising cells or microorganisms suspended within a nutrient growth medium; and rotating a first drive line and laterally spaced apart second drive line within the compartment of the container so as to cause the drive lines to twist into a helical configuration and mix the biological suspension.Type: GrantFiled: April 21, 2020Date of Patent: September 3, 2024Assignee: Life Technologies CorporationInventors: Nephi D. Jones, Christopher D. Brau
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Patent number: D1042846Type: GrantFiled: October 11, 2022Date of Patent: September 17, 2024Assignee: Shenzhen Yimi Life Technology Co., Ltd.Inventor: Yao Yi
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Patent number: D1048042Type: GrantFiled: December 11, 2020Date of Patent: October 22, 2024Assignee: Life Technologies CorporationInventors: Jing Gao, Alexander Roemer, Vrunda Sheth, Vidya Kudlingar, Dhirendrakumar Rai, Prateek Kumar