Patents Assigned to LIFE TECHNOLOGY CORPORATION
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Patent number: 12005412Abstract: A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.Type: GrantFiled: April 21, 2021Date of Patent: June 11, 2024Assignee: Life Technologies CorporationInventors: Nephi D. Jones, Brandon M. Knudsen, Whitt F. Woods
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Patent number: 12006543Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.Type: GrantFiled: March 30, 2021Date of Patent: June 11, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Guobin Luo, Marina Sedova, David Light, Ryan Jones, Mohammad Alanjary
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Patent number: 12006536Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.Type: GrantFiled: August 11, 2021Date of Patent: June 11, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Linda Lee, Sam Woo, Peter Ma
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Patent number: 11998910Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.Type: GrantFiled: August 26, 2021Date of Patent: June 4, 2024Assignee: Life Technologies CorporationInventors: Mark Reed, Zheng (Derek) Xing, David Marran, Earl Zergiebel
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Patent number: 12003145Abstract: A rotor assembly includes a rotor plate to rotate around a first axis, a bucket attached to the rotor plate and to rotate around a second axis, and a stop plate to rotate around the first axis between an open position and a closed position. When in the closed position, the stop plate engages the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly. The rotor assembly further includes a housing for a sensor array component, the housing disposed in the bucket and including a solution inlet, a solution outlet, a transfer basin, a solution retainer disposed between the solution outlet and the transfer basin, and a collection reservoir in fluid communication with the transfer basin. The solution inlet and the solution outlet to engage ports of a flow cell of a sensor array.Type: GrantFiled: February 2, 2023Date of Patent: June 4, 2024Assignee: Life Technologies CorporationInventor: Todd Roswech
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Patent number: 11999930Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.Type: GrantFiled: June 16, 2023Date of Patent: June 4, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Michael E. Goodwin, Nephi D. Jones
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Patent number: 11994528Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.Type: GrantFiled: December 15, 2020Date of Patent: May 28, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Alexandre Vlassov, Timothy Barta, Justin Inslee, Ethan Gentert, Robert Smith, Sara Thiele, Carolyn Adams, Kraig Kruger, Robert Schneider, Wade Lukianow, Jay Greco, Jason Jones, David Cerrone, Sumonto Ghosh, Brian Vajda, Andrew Basta, Allan Evans, Christopher Crowley, Robert Setterquist, Anne Cox, Mark Mayernick
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Patent number: 11996169Abstract: In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface (GUI) is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.Type: GrantFiled: June 24, 2022Date of Patent: May 28, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventor: Nivedita Sumi Majumdar
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Patent number: 11988585Abstract: Refractive index matching (RIM) formulations, their use for mounting biological specimens (e.g., cells and tissues) to a substrate, and methods of visualizing biological specimens embedded in RIM formulations are described.Type: GrantFiled: September 11, 2018Date of Patent: May 21, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Adam York, Eric Welch, Daniel Cash
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Patent number: 11982620Abstract: An optical detection system for a capillary electrophoresis instrument is disclosed. The system includes an ultraviolet (UV) source and an absorption measurement optical path. In an embodiment, the optical path comprises a first plurality of optical elements arranged to obtain a plurality of respective UV beamlets from a UV beam emitted by the UV source and to direct the respective UV beamlets transversely through respective capillaries of a plurality of capillaries and to an absorption detector positioned to detect respective signals for use in obtaining respective UV absorption measurements corresponding to the respective capillaries.Type: GrantFiled: March 17, 2020Date of Patent: May 14, 2024Assignee: Life Technologies CorporationInventor: Shaohong Wang
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Patent number: 11970787Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.Type: GrantFiled: February 4, 2021Date of Patent: April 30, 2024Assignee: Life Technologies CorporationInventors: Timothy Looney, Geoffrey Lowman, Lifeng Lin
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Patent number: 11958016Abstract: Dialysis devices include a frame defined by a plurality of sidewalls that are impermeable to a sample being dialyzed, a pair of dialysis membranes that are each associated with an opposing face of the plurality of sidewalls such that the plurality of sidewalls and the pair of dialysis membranes define a sample chamber, an outer shell surrounding at least a portion of the pair of dialysis membranes, and a cap selectively associated with the sample chamber. The cap can be selectively associated with the sample chamber via an attachment mechanism that is configured to provide aural and/or haptic feedback when the cap forms a tight association with the sample chamber. The cap can be a sensor cap having one or more probes for measuring at least one property of fluid inside and/or outside the sample chamber and a transmitter for transmitting data captured at the probe(s) to a destination device.Type: GrantFiled: June 28, 2019Date of Patent: April 16, 2024Assignees: Pierce Biotechnology, Inc., Life Technologies CorporationInventors: Kay Opperman, Barbara Kaboord, Aaron McBride, Greg Nagy, Sandro Klein, Joanna Geddes
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Patent number: 11961591Abstract: A method for detecting a tandem duplication in an FLT3 gene of a sample, includes mapping reads corresponding to targeted regions of exons of the FLT3 gene to a reference sequence. A partially mapped read includes a mapped portion, a soft-clipped portion and a breakpoint. Analyzing the partially mapped reads intersecting a column of the pileup includes detecting a duplication in the soft-clipped portion by comparing the soft-clipped portion to the mapped portion adjacent to the breakpoint; determining an insert size of the duplication in the soft-clipped portion; and assigning the partially mapped read to a category based on the insert size. Categories correspond to insert sizes. The categories are filtered and converted into features corresponding to the column. The features corresponding to one or more columns representing a same insert are merged to determine a location and size of a tandem duplication.Type: GrantFiled: November 30, 2018Date of Patent: April 16, 2024Assignee: Life Technologies CorporationInventor: Denis Kaznadzey
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Patent number: 11951474Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.Type: GrantFiled: June 21, 2021Date of Patent: April 9, 2024Assignee: Life Technologies CorporationInventors: Jonathan Schultz, David Marran
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Patent number: 11952495Abstract: The present disclosure provides for compounds of Formula (I), its corresponding compounds of Formula (II) or salts thereof and their use as fluorogenic pH sensors.Type: GrantFiled: October 11, 2021Date of Patent: April 9, 2024Assignee: Life Technologies CorporationInventors: Yi-Zhen Hu, Aimei Chen, Daniel Beacham, Chrisgen Vonnegut, Krishnamurthy Nacharaju
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Patent number: 11944945Abstract: A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment; a retainer coupled to the second end of the flexible bag, the retainer having a retention cavity formed thereon that communicates with the compartment of the flexible bag; and an elongated member having a first end rotatably coupled to the first end of the flexible bag and an opposing second end freely disposed within the retention cavity of the retainer so that the second end of the elongated member can rotate within the retention cavity relative to the retainer.Type: GrantFiled: November 27, 2020Date of Patent: April 2, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jeremy K. Larsen, Clinton C. Staheli, Brandon M. Knudsen
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Patent number: 11944975Abstract: A thermal cycler system comprises a sample block configured to receive a sample holder configured to receive a plurality of samples; an adaptor configured to surround a periphery of the sample block; and a drip pan configured to surround a periphery of the adaptor. The drip pan comprises one or more ejector mechanisms and one or more openings, the one or more ejector mechanisms configured to respectively extend through the one or more openings into contact with the sample holder in a state of the sample holder received by the sample block and the adaptor surrounding the periphery of the sample block.Type: GrantFiled: December 29, 2022Date of Patent: April 2, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Zeqi Tan, Wuh Ken Loh, Siew Yin Lee, Kuan Moon (Bernard) Boo
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Patent number: D1021947Type: GrantFiled: April 19, 2022Date of Patent: April 9, 2024Assignee: Life Technologies CorporationInventors: Sean Zimmerman, Scott Rickes
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Patent number: D1024319Type: GrantFiled: September 17, 2021Date of Patent: April 23, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Scott T. Hanks, Steven R. Kjar
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Patent number: D1025117Type: GrantFiled: March 1, 2022Date of Patent: April 30, 2024Assignee: Life Technologies CorporationInventors: Sean Zimmerman, Scott Rickes