Abstract: The invention relates to crosslinked soft tissue grafts and methods of use thereof. The invention also relates to methods of preparing the same.
Abstract: The invention is directed to a process for making a tissue repair implant having a porous sponge-like structure to repair bone, cartilage, or soft tissue defects by producing a connective tissue homogenate from one or more connective tissues; mixing the connective tissue homogenate with a carrier solution to produce a connective tissue carrier; optionally mixing one or more natural or synthetic bone fragements with said connective tissue carrier to produce a tissue repair mixture; freezing or freeze-drying the tissue repair mixture to produce a porous sponge-like structure and create a three-dimensional framework to entrap the natural or synthetic bone fragments, treating the frozen or freeze-dried porous sponge-like structure with one or more treatment solutions to produce a stabilized porous sponge-like structure. A crudely fragmented connective tissue from one or more connective tissues is optionally mixed with the tissue repair mixture before freezing or freeze-drying.
Type:
Grant
Filed:
August 7, 2008
Date of Patent:
September 15, 2015
Assignee:
LifeNet Health
Inventors:
Silvia S. Chen, Jingsong Chen, Lloyd Wolfinbarger, Jr.
Abstract: The present invention is directed toward recellularizing a devitalized cartilage graft with viable recellularizable cells in vivo, in situ, or in vitro to render the tissue vital. The present invention is also directed toward repairing a cartilage defect and implanting a cartilage graft into a human or animal by crafting a cartilage matrix into individual grafts, disinfecting and cleaning the cartilage graft, applying a pretreatment solution to the cartilage graft, removing cellular debris using an extracting solution to produce a devitalized cartilage graft, recellularizing the devitalized cartilage graft, implanting the cartilage graft into the cartilage defect with or without an insertion device, and sealing the implanted cartilage graft with recipient tissue. The devitalized cartilage graft is optionally stored between the removing cellular debris and the recellularizing steps.
Type:
Grant
Filed:
July 16, 2007
Date of Patent:
September 8, 2015
Assignee:
LifeNet Health
Inventors:
Silvia Sihui Chen, Xiaofei Qin, Jingsong Chen, Lloyd Wolfinbarger, Mark Moore, Rony Thomas, Alyce Linthurst Jones
Abstract: The present invention provides a plasticized dehydrated or freeze-dried bone and/or soft tissue product that does not require special conditions of storage, for example refrigeration or freezing, exhibits materials properties that approximate those properties present in normal hydrated tissue, is not brittle, does not necessitate rehydration prior to clinical implantation and is not a potential source for disease transmission. The invention replaces water in the molecular structure of the bone or soft tissue matrix with one or more plasticizers allowing for dehydration of the tissue, yet not resulting in an increase in brittleness of the plasticized product, and resulting in compressive and/or tensile properties similar to those of normal hydrated bone. Replacement of the chemical plasticizers by water prior to implantation is not required and thus, the dehydrated bone or soft tissue plasticized product can be placed directly into an implant site without significant preparation in the operating room.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
September 8, 2015
Assignee:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, Jr., Robert K. O'Leary, Billy G. Anderson
Abstract: Tissue repair compositions, particularly bone repair compositions, containing demineralized bone fragments and homogenized connective tissues, and methods for making the same. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form.
Type:
Application
Filed:
April 17, 2015
Publication date:
August 6, 2015
Applicant:
LifeNet Health
Inventors:
Leila Masinaei, Lloyd Wolfinbarger, JR.
Abstract: Tissue repair compositions, particularly bone repair compositions, containing (a) bone fragments and (b) homogenized connective tissue, and methods for making the same are provided. Some of the inventive tissue repair compositions contain a radioprotectant. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form. Kits for using such tissue repair compositions are also provided.
Abstract: Tissue repair compositions, particularly bone repair compositions, containing demineralized bone fragments and homogenized connective tissues, and methods for making the same. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form.
Type:
Grant
Filed:
June 30, 2011
Date of Patent:
May 19, 2015
Assignee:
LifeNet Health
Inventors:
Leila Masinaei, Lloyd Wolfinbarger, Jr.
Abstract: Tissue repair compositions, particularly bone repair compositions, containing (a) bone fragments and (b) homogenized connective tissue, and methods for making the same are provided. Some of the inventive tissue repair compositions contain a radioprotectant. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form. Kits for using such tissue repair compositions are also provided.
Abstract: The invention relates to truncated growth factors and variants thereof. The invention also relates to methods of making and using the truncated growth factors. The invention further relates to compositions including a protease and a growth factor comprising a bone morphogenic protein (BMP) or a variant thereof. The invention also relates to methods of using the composition.
Type:
Application
Filed:
September 2, 2012
Publication date:
March 5, 2015
Applicant:
LIFENET HEALTH
Inventors:
Xiaofei Qin, Silvia Chen, Jingsong Chen, James Clagett
Abstract: The present invention provides a plasticized dehydrated or freeze-dried bone and/or soft tissue product that does not require special conditions of storage, for example refrigeration or freezing, exhibits materials properties that approximate those properties present in normal hydrated tissue, is not brittle, does not necessitate rehydration prior to clinical implantation and is not a potential source for disease transmission. The invention replaces water in the molecular structure of the bone or soft tissue matrix with one or more plasticizers allowing for dehydration of the tissue, yet not resulting in an increase in brittleness of the plasticized product, and resulting in compressive and/or tensile properties similar to those of normal hydrated bone. Replacement of the chemical plasticizers by water prior to implantation is not required and thus, the dehydrated bone or soft tissue plasticized product can be placed directly into an implant site without significant preparation in the operating room.
Type:
Application
Filed:
February 28, 2014
Publication date:
June 26, 2014
Applicant:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, Jr., Robert K. O'Leary, Billy G. Anderson (deceased)
Abstract: The invention provides methodologies and apparatus for producing devitalized soft-tissue implants where the implant retains metabolically non-viable and/or reproductively non-viable cells, and preferably retains large molecular weight cytoplasmic proteins, such implants produced both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. A devitalized graft is produced by subjecting the tissue sample to an induced pressure mediated flow of an extracting solution, optionally followed by inducing a pressure mediated flow of a salt solution, then washing the tissue to produce the devitalized graft. The devitalized grafts produced are uniform and non-immunogenic. The inventive method allows for the production of multiple devitalized soft tissue implants, where processing time is significantly less than prior art processes and the number of implants produced per day is increased over prior art processes.
Type:
Application
Filed:
October 19, 2013
Publication date:
June 5, 2014
Applicant:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, Perry Lange, Alyce Linthurst-Jones, Eric Moore, Barry Nolf
Abstract: The invention provides methodologies and apparatus for producing acellular soft-tissue implants, both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. An acellular graft is produced by subjecting the tissue sample to an induced pressure mediated flow of an extracting solution, followed by inducing a pressure mediated flow of a treating solution, then washing the treated tissue to produce the acellular graft. The acellular grafts produced are uniform and nonimmunogenic. The inventive method allows for the production of multiple decellularized soft tissue implants, where processing time is significantly less than prior art processes and the number of implants produced per day is increased over prior art processes. In clinical use, the decellularized grafts produced exhibit significantly improved in long-tem durability and function.
Type:
Application
Filed:
October 31, 2013
Publication date:
March 6, 2014
Applicant:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, JR., Peny Lange, Alyce Linthurst-Jones, Eric Moore, Barry Naif
Abstract: The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them.
Type:
Application
Filed:
September 18, 2013
Publication date:
January 16, 2014
Applicant:
LifeNet Health
Inventors:
Roberto Bracone, Jingsong Chen, Silvia Chen, James A. Clagett, Marilyn Gatin, Daniel Osborne
Abstract: The invention relates to cell support compositions comprising a basement membrane extract isolated from cardiac or smooth muscle. The invention also relates to methods of using the cell support compositions for supporting cellular functions.
Abstract: The invention is directed to an apparatus for producing demineralized osteoinductive bone. The apparatus demineralizes bone by subjecting bone, including, for example, ground bone, bone cubes, chips, strips, or essentially intact bone, to either a rapid high volume pulsatile acidification wave process or to a rapid continuous acid demineralization process. The pulsatile acidification wave process includes subjecting bone to two or more rapid pulse/drain cycles in which one or more demineralizing acids is rapidly pulsed into a vessel containing bone, and after a desired period of time, is rapidly drained from the vessel. The continuous acid demineralization process includes subjecting bone to a continuous exchange of demineralizing acid solution in which the demineralizing acid solution is recirculated from the container holding the bone through an ion exchange media.
Abstract: The invention provides methodologies and apparatus for producing a cellular soft-tissue implants, both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. An acellular graft is produced by subjecting the tissue sample to an induced pressure mediated flow of an extracting solution, followed by inducing a pressure mediated flow of a treating solution, then washing the treated tissue to produce the acellular graft. The acellular grafts produced are uniform and nonimmunogenic. The inventive method allows for the production of multiple decellularized soft tissue implants, where processing time is significantly less than prior art processes and the number of implants produced per day is increased over prior art processes. In clinical use, the decellularized grafts produced exhibit significantly improved in long-term durability and function.
Type:
Grant
Filed:
October 8, 2010
Date of Patent:
November 5, 2013
Assignee:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, Jr., Perry Lange, Alyce Linthurst Jones, Eric Moore, Barry Nolf
Abstract: The invention provides methodologies and apparatus for producing devitalized soft-tissue implants where the implant retains metabolically non-viable and/or reproductively non-viable cells, and preferably retains large molecular weight cytoplasmic proteins, such implants produced both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. A devitalized graft is produced by subjecting the tissue sample to an induced pressure mediated flow of an extracting solution, optionally followed by inducing a pressure mediated flow of a salt solution, then washing the tissue to produce the devitalized graft. The devitalized grafts produced are uniform and non-immunogenic. The inventive method allows for the production of multiple devitalized soft tissue implants, where processing time is significantly less than prior art processes and the number of implants produced per day is increased over prior art processes.
Type:
Grant
Filed:
October 28, 2003
Date of Patent:
October 22, 2013
Assignee:
LifeNet Health
Inventors:
Lloyd Wolfinbarger, Jr., Perry Lange, Alyce Linthurst-Jones, Eric Moore, Barry Nolf