Abstract: Methods for detection of the activity of proteolytic enzymes, particularly isopeptidases, are disclosed.

Abstract: Synthetic protease substrates and methods which facilitate the identification of substrates of a protease, particularly ubiquitin, ubiquitin-like, or proteasome protein are provided.

Abstract: Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.

Abstract: Methods for detection of the activity of proteolytic enzymes, particularly isopeptidases, are disclosed.

Abstract: Methods for detection of the activity of proteolytic enzymes, particularly isopeptidases, are disclosed.

Abstract: Recombinant Saccharomyces cerevisiae produced by transformation with heterologous polynucleotide sequences coding for xylulokinase (XKS) from Yersinia pestis and xylose isomerase (XI) are capable of xylose utilization. The transformants express these heterologous polynucleotides at a sufficient functional level to grow aerobically on xylose as the sole carbon source. Further transformation of the recombinant yeasts to overexpress one or more of the S cerevisiae genes PIP2, IMG2, MAK5, VPS9, COX10, ALE1, CDC7, and MMS4, permits the yeast to grow anaerobically on xylose as the sole carbon source. When grown under anaerobic conditions on a culture medium comprising both glucose and xylose, the transformed yeast exhibit increased ethanol productivity, with the yeast growing on the xylose to increase their biomass and fermenting the glucose to ethanol.

Abstract: The inventive subject matter relates to novel compositions, methods, and kits for enhancing the expression, solubility, and isolation of heterologous proteins. Further, the inventive subject matter relates to methods for generating proteins with novel N-terminal amino acids, unlike wild-type proteins which always are translated from mRNA with methionine at the N-terminus.

Abstract: Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Abstract: Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Abstract: Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Abstract: A yeast based transactivation assay for gender sorting is disclosed.

Abstract: Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Abstract: A yeast-based system and methods of use therefore are provided for identifying new molecules which activate nuclear receptors in a ligand-dependent fashion. In a preferred embodiment, a method is provided utilizing ecdysone receptor, USP and GRIP I encoding expression vectors which may be used to advantage for screening new and useful insecticidal compounds, detecting insecticidal residues as well as to regulate expression of a gene of interest in a host in a ligand-dependent manner.

Abstract: The present invention relates to an apparatus for monitoring the intracranial pressure of a patient. The apparatus includes an implantable sensor implanted in the skull of a patient. The implantable sensor senses pressure and outputs a signal indicative of the pressure in the patient's skull. The apparatus further includes a probe receiving the signal output by the implantable sensor when the probe is positioned near the implantable sensor. The probe outputs a signal indicative of the pressure sensed by the implantable sensor. An interrogation circuit is provided for receiving the signal output by the probe and automatically outputting a digital signal proportional to the pressure sensed by the implantable sensor.