Abstract: The invention describes a method for utilizing the CRISPR-Cas system to edit any gene of interest present on a plasmid. The method uses CRISPR tool to engineer enzymes for better activity by allowing a cell to undergo specific and random mutations. Described methods include newly designed, engineered and modified vector systems, which encodes single or multiplex gene targets and Cas9/deaminase Cas9 proteins. The invention is useful for single or multiple gene editing for industrial applications such as to edit genes encoding antibiotics, therapeutic proteins or any important industrial enzymes. The invention is a quick and efficient tool for creating enzyme variant libraries containing a vast range of permutation and combination of mutation that will be assayed for highest activity. Hot spots will be identified on gene of interest which will aid in generating mutations in these places. These mutations can be rationalized in specific places or random single base substitutions.

Abstract: The present invention relates to modified bacteria for introducing desired mutations in target sequences in plant cells, wherein the bacteria has reduced VirD5 activity and carries site-specific DNA editing machinery. The invention also provides methods for generating plant cells, plant parts, plants or populations thereof using such bacteria.

Abstract: A stripper composition and a method for stripping a photoresist are provided. The stripper composition includes an ether-alcohol-based organic solvent (A), another organic solvent (B), an alkaline substance (C), a substrate corrosion inhibitor (D), and water (E). The another organic solvent (B) does not include the ether-alcohol-based organic solvent. The alkaline substance (C) includes an organic base (C1), an inorganic base (C2), or a combination thereof. Based on a total usage amount of 100 parts by weight of the stripper composition, a usage amount of the ether-alcohol-based organic solvent (A) is 7 parts by weight to 70 parts by weight, and a usage amount of the substrate corrosion inhibitor (D) is greater than 0 part by weight and less than 18 parts by weight.

Abstract: Provided are a pipe attachment structure (1) and a bracket (20) that minimize breakage of a pipe (10). This pipe attachment structure is mounted on a moving body and a pipe through which a fluid flows is attached to a predetermined component via a bracket, wherein the bracket has a flange part (23) formed into a flange by bending the bracket such that of the portion that attaches to the outer peripheral surface of the pipe, the end part in the flowing direction of the fluid separates from the outer peripheral surface of the pipe.

Abstract: A thermal conductivity sensor for measuring a concentration of a gas, the sensor comprising: a substrate portion; an intermediate layer disposed on the substrate portion; a semiconductor layer disposed on the intermediate layer, and a dielectric layer comprising a dielectric membrane, the dielectric membrane provided with a heater; wherein the dielectric membrane is located over a gap, the gap being located in the semiconductor layer. Methods for manufacturing a thermal conductivity sensor are also described.

Abstract: Provided is a mixed reality device including a Fourier 4F optical architecture and a design of folding a light path to display images of different depths. In addition to achieving real-time multi-depth and mixed reality display effects, it may also have a wide field of view and effectively avoid a vergence-accommodation conflict.

Abstract: An inertial sensor includes a dual atom interferometer. A moving standing light wave generation device in the dual atom interferometer generates M (3?M) moving standing light waves. Each of the M moving standing light waves is generated by a pair of counter-propagating laser beams. The moving standing light wave generation device adjusts two-photon detuning of a pair of counter-propagating laser beams corresponding to each of N (2?N<M) moving standing light waves out of the M moving standing light waves such that the difference between an interferometric phase corresponding to one atomic beam obtained by interfering with the M moving standing light waves and an interferometric phase corresponding to the other atomic beam obtained by interfering with the M moving standing light waves stays constant.

Abstract: A substrate processing method includes: (A) applying a resist liquid on a substrate to form a resist film, (B) performing auxiliary exposure processing of irradiating the resist film with light having a desired wavelength, separately from exposure processing of transferring a pattern of a mask onto the resist film, (C) supplying a developer to the resist film after the exposure processing and the auxiliary exposure processing to form a resist pattern, (D) etching an etching target layer on the substrate using the resist pattern as a mask, and (E) correcting an in-plane distribution of an exposure amount in the auxiliary exposure processing in (B), and performing the correction based on a result of (D) when (A) to (D) are performed under conditions before the correction.

Abstract: The present invention provides a rapid method using whole blood samples for determining the severity of COVID-19 disease arising from SARS-CoV-2 infection and monitoring progression and treatment of the disease relying on determination of the functionality of leukocytes (predominately neutrophils) to exhibit challenge-induced superoxide anion production with quantification by chemiluminescent measurement.

Abstract: A touch panel and a touch display device are provided. The touch panel includes an electrode layer. The electrode layer includes: a plurality of conducting regions arranged in parallel and at intervals; a non-conducting region located between adjacent conducting regions, where the non-conducting region includes an edge region close to a conducting region and a central region far away from the conducting region, and the edge region is adjacent to the central region; and a plurality of suspending blocks located in the non-conducting region, where cutting grooves are provided between the suspending block close to the conducting region and the conducting region, and between adjacent suspending blocks, and a density of the cutting grooves located in the edge region is greater than that of the cutting grooves located in the central region.

Abstract: A web scaping system configured with artificial intelligence and image object detection. The system processes a web page with a neural network to perform object detection to obtain structured data, including text, image and other kinds of data, from web pages. The neural network allows the system to efficiently process visual information (including screenshots), text content and HTML structure to achieve good quality and decrease extraction time.

Abstract: A storage unit stores one of flow and distance matrices for an assignment problem having an evaluation function represented by a matrix operation of the flow and distance matrices, and a processing unit selects first and second entities from entities to be assigned to destinations, reads at least one first matrix element corresponding to the first and second entities from the stored flow or distance matrix, generates at least one second matrix element for the other of the flow and distance matrices, which is a patterned matrix, based on the first and second entities, calculates a change in the value of the evaluation function resulting from an assignment change of changing the destination of the first or second entity, using the first and second matrix elements, determines based on the change whether to allow the assignment change, and updates an assignment state when determining to allow the assignment change.

Abstract: A medium storing a program for causing a computer to execute: specifying a package to be delivered by a first moving body from a first base to a second base in a first plan; specifying a third base where delivery of the package from the first base to the third base is allocatable to a second moving body in the first plan and where delivery of the package from the third base to the second base is allocatable to a third moving body in the first plan; and creating a second plan by replacing the delivery of the package from the first base to the second base by the first moving body with the delivery of the package from the first base to the third base by the second moving body and the delivery of the package from the third base to the second base by the third moving body.

Abstract: Provided is a state detection apparatus, a state detection method, a generation method of a learning model, and a recording medium, which can be expected to accurately detect a state of a target apparatus such as a semiconductor manufacturing apparatus. The state detection apparatus according to the present embodiment includes: a first acquirer that acquires input data of a target apparatus and observed data of an operation of the target apparatus; a determinator that determines parameters of an estimation model that estimates the observed data from the input data based on the input data and the observed data acquired by the first acquirer; and a detector that detects a state of the target apparatus based on the parameters determined by the determinator.

Abstract: The present invention provides solution to the above-mentioned problem in the art by providing a system and a method for efficiently identifying from a medical domain a plurality of entities that may be designed and engineered for a diagnostic reasoning system or symptom checker. The identified plurality of entities forms nodes of a knowledge graph and may be interconnected to each other with edges representing finely curated weights. The curated weights may form a dynamic repository that can constantly evolves to best reflect the latest medical knowledge. Apart from diseases and symptoms, the system is also capable of extending and scaling to other dimensions of medical knowledge. The knowledge graph thus developed is fundamental to the entire diagnostic system and is used by various components of the diagnostic engine to power the AI-based reasoning.

Abstract: A battery pack includes: a cell module, including at least one cell; a first collection module, including at least one first collection unit, the first collection unit being disposed within the cell, and being configured to collect first operational data of the cell; a data processing module, connected to the first collection unit, configured to perform thermal runaway detection on the cell based on the first operational data to obtain a first detection result; a second collection module, disposed outside the cell module, configured to be activated when the first detection result indicates that any one of the cell has a thermal runaway risk, and collect second operational data of the cell after activation; the data processing module is further connected to the second collection module, and further configured to perform secondary detection based on the second operational data to determine whether the cell has a thermal runaway risk.

Abstract: An end cap, a battery cell, a battery, and an electrical device are disclosed. The end cap includes: an end cap body, where a hole is created on the end cap body; a first connection structure, including a first metal, where the first connection structure is disposed on an inner side of the hole and configured to be welded to an electrode terminal located in the hole; and a second connection structure, including a second metal. The second connection structure is connected to the first connection structure. The second connection structure is configured to be welded to a first sealing element designed for sealing the hole.

Abstract: A separator includes a porous substrate and a porous coating. The porous coating is disposed on at least one surface of the porous substrate. The porous coating includes inorganic particles and a binder. The binder includes a first binder. The first binder includes a metal element. The separator of this application is excellent in thermal safety stability and mechanical stability, mainly manifested in that, when a rupture hole is generated on the separator by thermally puncturing the separator by using a round needle with a diameter of R heated to 500° C., a maximum value of a distance between any two points on an edge of the rupture hole is r in a case that the two points are connected to form a line and the distance between the two points is calculated, satisfying: 400 ?m?R?1000 ?m, and 0.9?r/R?5.

Abstract: A separator includes a porous substrate and a porous coating. The porous coating is disposed on at least one surface of the porous substrate. The porous coating includes inorganic particles and a binder. The separator of this application exhibits excellent thermal safety stability and mechanical stability, mainly manifested in that the Modulus of the separator at temperature T is P, satisfying: 100° C.?T?180° C., and 108 Pa?P?109 Pa.

Abstract: Methods and apparatuses for multi-DCI multi-TRP based UL transmission in unified TCI framework are disclosed. In one embodiment, a UE comprises a processor; and a receiver coupled to the processor, wherein the processor is configured to receive, via the receiver, an MAC CE including a CORESET pool ID field to activate one or multiple UL or joint TCI states among configured UL or joint TCI states; and receive, via the receiver, a DCI indicating one UL or joint TCI state when multiple UL or joint TCI states are activated.