Abstract: This invention discloses dialysis devices, related housings, related membranes, related kits, and related methods.

Abstract: This invention discloses dialysis devices, related housings, related membranes, related kits, and related methods.

Abstract: This invention discloses dialysis devices, related housings, related membranes, related kits, and related methods.

Abstract: The invention features linker molecules that have at one terminus a amino-protecting group and at the other terminus a phosphorous activating group, typically phosphoramidite. The linker molecules can be used, for example, to produce amino-modified linkers that space an oligonucleotide from a solid support. The invention also features an amino-protected nucleotide that includes an activated phosphorous group such as a phosphoramidite for the production of a 5? amino-modified oligonucleotide. The invention further provides a phthalimido-modified support that can be used to synthesize a polynucleotide that includes an amino group at the 3? terminus.

Abstract: Nucleotide preparations are transferred from a first substrate to a second substrate. One transfer method includes forming a patterned dry particulate deposition on a first substrate; positioning the first substrate in apposition to a second substrate; and transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate. The method can be used to form an array of nucleic acids.

Abstract: Disclosed is an insoluble support that can be used, for example, for producing replicates of sample nucleic acids. The support includes a plurality of attached oligonucleotides that include a prokaryotic promoter sequence and a target annealing sequence 3? of the promote. The proximal end of the promoter sequence is spaced from the insoluble support by a distance greater than 10 nm.

Abstract: Disclosed are compositions that include triboelectrically chargeable nucleotide particles of less than 50 ?m diameter and carrier particles. In one example, a substrate is selectively patterned with the compositions, e.g., by transfer from a selectively charged surface. The compositions can be used to synthesize nucleic acid arrays.

Abstract: Disclosed is a method of producing replicates of sample nucleic acids. The method can include providing an insoluble support comprising attached oligonucleotides, annealing sample nucleic acids to the attached oligonucleotides; constructing template nucleic acids by extending the attached oligonucleotides using a polymerase; and transcribing the template nucleic acids to produce RNA replicates of the sample nucleic acids The attached oligonucleotides comprise a promoter sequence and a target annealing sequence, and (2) the proximal end of the promoter sequence is spaced from the insoluble support by a predetermined distance.

Abstract: Disclosed is a method of producing replicates of sample nucleic acids. The method can include providing an insoluble support comprising attached oligonucleotides, annealing sample nucleic acids to the attached oligonucleotides; constructing template nucleic acids by extending the attached oligonucleotides using a polymerase; and transcribing the template nucleic acids to produce RNA replicates of the sample nucleic acids The attached oligonucleotides comprise a promoter sequence and a target annealing sequence, and (2) the proximal end of the promoter sequence is spaced from the insoluble support by a predetermined distance.

Abstract: Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5′ end of the nucleotide is tetherable to the linking group and the protected C-3′ end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3′ primer extension.

Abstract: Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5′ end of the nucleotide is tetherable to the linking group and the protected C-3′ end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3′ primer extension.

Abstract: Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5′ end of the nucleotide is tetherable to the linking group and the protected C-3′ end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3′ primer extension.