Abstract: An analog to digital signal converter includes an integrator and a sample and hold circuit. Precisely repeatable timing signals for both the integrator and sample and hold circuit are provided by a microprocessor. The device provides low pass filtering without the phase lag normally associated with linear filters. The device allows one set of data to be acquired while the previous set of data is being digitized. The device is especially applicable to analysis of data generated by a spectrophotometer.

Abstract: A method and device for smoothly rotating a diffraction grating in a spectrophotometer by means of a galvanometer includes modifications in the software in the microprocessor which controls the galvanometer. The software modifications cause the galvanometer to rotate the grating in a series of discrete steps that stimulate a smooth minimum jerk rotation. The amount of rotation in each discrete step is calculated by the microprocessor.

Abstract: An electrophoresis instrument includes a capillary tube mounted in an air cooled cartridge. The cartridge also supports a spherical lens which is part of the optical detection apparatus. The cartridge rests in a manifold which includes the sample and buffer reservoirs. The temperature of the capillary tube is controlled by measuring the electrical resistance of the capillary tube during the electrophoresis process and then cooling or heating the cartridge by circulating temperature controlled air over the tube. The optical path associated with the instrument is a fiber optic bundle bifurcated close to dual detectors into a reference arm and a sample arm so as to provide similar reference and sample optical paths. The instrument may be used for temperature control for buffer gradient electrophoresis and also, a neutral marker for determining electro-osmotic flow may be detected.

Abstract: Absorbance and wavelength data which is generated by a liquid chromatography apparatus is orthogonalized and resampled by a cubic splining technique in order to provide a smooth spectra curve showing absorbance versus wavelength, with the time factor eliminated. This has the advantage of eliminating from the data any changes in the sample over time. The method results in the orthogonalization with respect to time of the data. The invention is applicable to analysis of data other than liquid chromatography data. Higher or lower order curve fitting techniques may be used instead of the cubic splining analysis method.

Abstract: In a spectrophotometer application where high speed positioning is critical, a galvanometer in conjunction with a microprocessor controlled hybrid digital/analog servo system is used to rotate a diffraction grating for wavelength selection. A table containing digital position information for all wavelengths is accessed by the microprocessor to perform wavelength changes. The use of the table permits the determination of grating position to yield a desired wavelength for a system where the axis of rotation does not intersect a point on the surface of the diffraction grating. That is, the diffraction grating can be rotated about an axis coinciding with its center of gravity.

Abstract: An improved mounting for a rotating diffraction grating assembly as used in a spectrophotometer directly connects the grating to the galvanometer that rotates the grating. The galvanometer is gimbally mounted on a plate so that its position, and that of the grating, can be adjusted so that the plane of dispersion of the grating passes through a desired point when the grating is rotated.