Abstract: An analog to digital signal converter includes an integrator and a sample and hold circuit. Precisely repeatable timing signals for both the integrator and sample and hold circuit are provided by a microprocessor. The device provides low pass filtering without the phase lag normally associated with linear filters. The device allows one set of data to be acquired while the previous set of data is being digitized. The device is especially applicable to analysis of data generated by a spectrophotometer.

Abstract: There is disclosed herein a cell assembly for a spectrophotometric analysis or detection of a substance within a small sample volume. The assembly includes a cell body with a small sample bore, along with windows engaging said body to allow radiation to pass through the sample bore and the windows. A sealing arrangement is provided for each window via a sealing washer which is loaded by two means. An inner portion of the sealing washer engages the window and is loaded by a first spring loaded assembly, such as a Belleville washer and plunger. An outer portion of the sealing washer is separately loaded by a ring pressed against the cell body.

Abstract: A method and device for smoothly rotating a diffraction grating in a spectrophotometer by means of a galvanometer includes modifications in the software in the microprocessor which controls the galvanometer. The software modifications cause the galvanometer to rotate the grating in a series of discrete steps that stimulate a smooth minimum jerk rotation. The amount of rotation in each discrete step is calculated by the microprocessor.

Abstract: An electrophoresis instrument includes a capillary tube mounted in an air cooled cartridge. The cartridge also supports a spherical lens which is part of the optical detection apparatus. The cartridge rests in a manifold which includes the sample and buffer reservoirs. The temperature of the capillary tube is controlled by measuring the electrical resistance of the capillary tube during the electrophoresis process and then cooling or heating the cartridge by circulating temperature controlled air over the tube. The optical path associated with the instrument is a fiber optic bundle bifurcated close to dual detectors into a reference arm and a sample arm so as to provide similar reference and sample optical paths. The instrument may be used for temperature control for buffer gradient electrophoresis and also, a neutral marker for determining electro-osmotic flow may be detected.

Abstract: Absorbance and wavelength data which is generated by a liquid chromatography apparatus is orthogonalized and resampled by a cubic splining technique in order to provide a smooth spectra curve showing absorbance versus wavelength, with the time factor eliminated. This has the advantage of eliminating from the data any changes in the sample over time. The method results in the orthogonalization with respect to time of the data. The invention is applicable to analysis of data other than liquid chromatography data. Higher or lower order curve fitting techniques may be used instead of the cubic splining analysis method.

Abstract: There is disclosed herein a cell assembly for use in spectrophotometric analysis or detection of a substance within a small sample volume, such as in a capillary sample cell. The assembly comprises a transparent lens having the shape of a ball, sphere or cylinder, referred to as a "ball lens," and which is placed in direct contact or close proximity to the exterior wall of the capillary cell. This lens and cell are mounted together, any may form a removable cell assembly. The small volume may be contained within the ball lens itself. The ball lens serves to concentrate and direct light into the capillary cell. The arrangement also significantly reduces the sensitivity to alignment of replaceable cells thereby permitting easier mounting and replacement of such cells.

Abstract: In a spectrophotometer application where high speed positioning is critical, a galvanometer in conjunction with a microprocessor controlled hybrid digital/analog servo system is used to rotate a diffraction grating for wavelength selection. A table containing digital position information for all wavelengths is accessed by the microprocessor to perform wavelength changes. The use of the table permits the determination of grating position to yield a desired wavelength for a system where the axis of rotation does not intersect a point on the surface of the diffraction grating. That is, the diffraction grating can be rotated about an axis coinciding with its center of gravity.

Abstract: An improved mounting for a rotating diffraction grating assembly as used in a spectrophotometer directly connects the grating to the galvanometer that rotates the grating. The galvanometer is gimbally mounted on a plate so that its position, and that of the grating, can be adjusted so that the plane of dispersion of the grating passes through a desired point when the grating is rotated.

Abstract: A servo amplifier for d-c servo motors which converts the error signal into pulses which are amplified in a saturating amplifier to drive the motor. Both the duty cycle and the repetition rate of the pulses vary with the error signal. At large error signals the duty cycle is 100 percent, i.e., "full on"; at small error signals the duty cycle is low and the pulses spaced far apart. As the system approaches balance the pulses become shorter and less frequent, and the motor slows. The circuit comprises a signal integrator which drives two comparators, one for positive signals and the other for negative. The integrator generates a ramp voltage in a direction opposite to the polarity of its input, and is reset automatically by reset pulses of controlled and variable length when a comparator set point is reached. The reset pulses are the pulses which are amplified to drive the servo motor. In this system, reducing the gain does not produce a widened dead band, but only increases the time required for rebalance.