Abstract: An improvement in the process for the determination of the concentration of adenosinetriphosphate (ATP) in ATP-converting reactions wherein the concentration is a function of the intensity of light emitted when a sample to be assayed is contacted with a bioluminescent reagent based on D-luciferin, luciferase and magnesium ions or certain other metal ions, the emitted light resulting from ATP and D-luciferin becoming bound to the luciferase, comprises the use of one or more competitive inhibitors such as a D-luciferin analogue, or analogues.