Abstract: The present invention relates generally to adjuvants, and in particular to methods and products utilizing a synergistic combination of immunostimulatory oligonucleotides having at least one unmethylated CpG dinucleotide (CpG ODN) and a non-nucleic acid adjuvant. Such combinations of adjuvants may be used with an antigen or alone. The present invention also relates to methods and products utilizing immunostimulatory oligonucleotides having at least one unmethylated CpG dinucleotide (CpG ODN) for induction of cellular immunity in infants.
Type:
Application
Filed:
December 18, 2001
Publication date:
November 7, 2002
Applicant:
Loeb Health Research Institute at the Ottawa Hospital
Inventors:
Heather L. Davis, Joachim Schorr, Arthur M. Krieg
Abstract: The present invention shows that DNA vaccine vectors can be improved by removal of CpG-N motifs and optional addition of CpG-S motifs. In addition, for high and long-lasting levels of expression, the optimized vector should include a promoter/enhancer that is not down-regulated by the cytokines induced by the immunostimulatory CpG motifs. Vectors and methods of use for immunostimulation are provided herein. The invention also provides improved gene therapy vectors by determining the CpG-N and CpG-S motifs present in the construct, removing stimulatory CpG (CpG-S) motifs and/or inserting neutralizing CpG (CpG-N) motifs, thereby producing a nucleic acid construct providing enhanced expression of the therapeutic polypeptide. Methods of use for such vectors are also included herein.
Type:
Grant
Filed:
May 20, 1998
Date of Patent:
January 15, 2002
Assignees:
University of Iowa Research Foundation, Loeb Health Research Institute at the Ottawa Hospital, Coley Pharmaceutical GmbH
Inventors:
Arthur M. Krieg, Heather L. Davis, Tong Wu, Joachim Schorr
Abstract: The present invention relates to methods of immunization of aquaculture species by introducing DNA expression systems into the aquaculture species. Such DNA expression systems preferably include DNA sequences encoding polypeptides of pathogens of species of aquaculture. The present invention also relates to methods of administration of DNA expression systems into aquaculture. Such methods include injection, spray, and immersion techniques. The methods of this invention are useful for prophylactic vaccination or therapeutic immunization of fin-fish, shellfish, or other aquatic animals against infectious diseases.
Type:
Grant
Filed:
July 14, 1998
Date of Patent:
January 30, 2001
Assignee:
Loeb Health Research Institute at The Ottawa Hospital