Patents Assigned to Longhorn Vaccines & Diagnostics LLC
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Patent number: 9080204Abstract: Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types.Type: GrantFiled: December 30, 2011Date of Patent: July 14, 2015Assignee: Longhorn Vaccines and Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20150182606Abstract: The present invention is directed to compositions and methods for preventing and/or treating diseases and disorders of patients caused by non-Staphylococcal microorganisms. In particular, compositions and methods contain lysostaphin, altered forms of lysostaphin as compared to wild-type, and synergistic combinations of lysostaphin plus additional conventional treatments such as other enzyme, antibiotic and/or antibody treatment. The invention is also directed to detecting and identifying altered forms of lysostaphin that possess increased efficacy against infections as compared to wild-type lysostaphin, and forms that generate a minimal or no immune response in a patient. The invention is also directed to method of manufacturing lysostaphin and altered forms of lysostaphin, and compositions that direct the lysostaphin to the site of the infection such as aerosolized nanoparticles.Type: ApplicationFiled: December 29, 2014Publication date: July 2, 2015Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Gerald W. Fischer, Richard F. Schuman
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Publication number: 20150056609Abstract: Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria.Type: ApplicationFiled: October 29, 2014Publication date: February 26, 2015Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Luke T. Daum, Gerald W. Fischer
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Patent number: 8821885Abstract: The invention is directed to immunogenic compositions, and methods for their use in the formulation and administration of therapeutic and prophylactic pharmaceutical agents. In particular, the invention provides immunogenic compositions and methods for preventing, treating, and/or ameliorating microbial infection, including, for example, influenza, or one or more symptoms thereof.Type: GrantFiled: August 27, 2008Date of Patent: September 2, 2014Assignee: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20140193804Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for presenting the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: ApplicationFiled: January 7, 2014Publication date: July 10, 2014Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Gerald W Fischer, Luke T. Daum
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Noninterfering Multipurpose Compositions for Collecting, Transporting and Storing Biological Samples
Publication number: 20140186821Abstract: The invention is directed to compositions and methods for collecting, transporting, and storing microorganisms obtained from samples of biological, clinical, forensic, and environmental origin. Compositions preserve the viability of the collected organisms, permit long-term storage, and are compatible with subsequent manipulation including propagation and culture of collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids and macromolecules. When the compositions containing microorganisms and polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and/or characterize select microbes and their components, when compared to conventional microbial transport media that contain interfering substance(s).Type: ApplicationFiled: December 20, 2013Publication date: July 3, 2014Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Luke T. Daum, Gerald W. Fischer -
Patent number: 8669240Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for presenting the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: GrantFiled: March 19, 2013Date of Patent: March 11, 2014Assignee: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Patent number: 8652782Abstract: Disclosed are compositions and methods for isolating, detecting, amplifying, and quantitating Mycobacterium-specific nucleic acids in a sample. Also disclosed are compositions and diagnostic kits comprising Mycobacterium IS6110-specific oligonucleotide amplification primers and labeled oligonucleotide detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more tubercular pathogens, including Mycobacterium tuberculosis, in particular, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.Type: GrantFiled: April 26, 2011Date of Patent: February 18, 2014Assignee: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20140038174Abstract: The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and Mycobacterium tuberculosis, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.Type: ApplicationFiled: October 8, 2013Publication date: February 6, 2014Applicant: LONGHORN VACCINES AND DIAGNOSTICS, LLCInventors: GERALD W. FISCHER, LUKE T. DAUM
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Publication number: 20140030703Abstract: The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and Mycobacterium tuberculosis, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.Type: ApplicationFiled: October 8, 2013Publication date: January 30, 2014Applicant: LONGHORN VACCINES AND DIAGNOSTICS, LLCInventors: GERALD W. FISCHER, LUKE T. DAUM
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Publication number: 20140011187Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: ApplicationFiled: March 19, 2013Publication date: January 9, 2014Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20130302789Abstract: Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria.Type: ApplicationFiled: May 9, 2013Publication date: November 14, 2013Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Luke T. Daum, Gerald W. Fischer
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Compositions and Methods for the Collection and Isolation of Nucleic Acids from Biological Specimens
Publication number: 20130260369Abstract: The invention is directed to tools, compositions and methods for collecting, storing, transporting, isolating and detecting macromolecules such as nucleic acid sequences obtained from specimens. The compositions are one-step formulations for killing or inactivating pathogens, inactivating enzymes, and releasing nucleic acids from the specimens that are prepared for further processing and/or analysis. In particular, the invention provides a single, one-step, sample collection and transport formulation that facilitates the concentration, extraction, isolation and analysis of nucleic acids, genes and genomes.Type: ApplicationFiled: March 15, 2013Publication date: October 3, 2013Applicant: Longhorn Vaccines and Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum -
Publication number: 20130195909Abstract: The invention relates to composite antigens comprising a peptide with contiguous amino acid sequence derived from a plurality of antigenic epitopes of one or more pathogens that induces an immune response in a mammal that is protective against infection by the one or more pathogens. In addition, the invention relates to vaccines comprising composite antigens and to method for treating and preventing an infection.Type: ApplicationFiled: January 25, 2013Publication date: August 1, 2013Applicant: Longhorn Vaccines and Diagnostics. LLCInventor: Longhorn Vaccines and Diagnostics, LLC
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Patent number: 8415330Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: GrantFiled: October 1, 2012Date of Patent: April 9, 2013Assignee: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20130040288Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: ApplicationFiled: October 1, 2012Publication date: February 14, 2013Applicant: Longhorn Vaccines & Diagnostics, LLC.Inventor: Longhorn Vaccines & Diagnostics, LLC.
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Patent number: 8293467Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: GrantFiled: December 20, 2011Date of Patent: October 23, 2012Assignee: Longhorn Vaccines & Diagnostics LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20120115126Abstract: Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types.Type: ApplicationFiled: December 30, 2011Publication date: May 10, 2012Applicant: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20120107799Abstract: An extraction apparatus and methods adapted to extract and preferably also isolate structures of interest from a test sample. The extraction apparatus contains a volume-dispensing mechanism that facilitates control over the injection of a sample suspected of containing a structure of interest into a filtration vessel that contains a membrane filter that associates with the structure of interest, preferably by adsorbing or binding thereto, and a collection container associated therewith. Methods of extracting, isolating, testing, and instructions regarding such methods are also included.Type: ApplicationFiled: October 29, 2010Publication date: May 3, 2012Applicant: Longhorn Vaccines & Diagnostics LLC.Inventor: Luke T. Daum
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Publication number: 20120100529Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: ApplicationFiled: December 20, 2011Publication date: April 26, 2012Applicant: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum