Abstract: With respect to molecular biology, medicine, and biotechnology, provided is a method related to the performance of Polymerase Chain Reaction. The method includes using the device for identification of nucleic acids containing a microchip with at least one reaction zone on its surface. The microchip contains a heat-conducting substrate made of aluminum while each reaction zone is separated from the heat-conducting substrate by a layer of the passivating material covalently bound to the surface of the heat-conducting substrate while over the layer of the passivating material one or several dried components of the polymerase chain reaction is placed. In this method the ratio of the aggregate thermal mass of the microchip to thermal conductance of the microchip substrate does not exceeding 0.04 s. The technical result is reduced duration of the analysis, higher reliability, accuracy, efficiency and cost-effectiveness of the analysis.
Type:
Grant
Filed:
October 8, 2009
Date of Patent:
April 25, 2017
Assignee:
Lumex Instruments Limited
Inventors:
Alexander Anatolevich Stroganov, Maxim Nikolaevich Slyadnev
Abstract: The invention relates to analytical chemistry. In the analyser, mercury placed in a spectral lamp is enriched with a mercury isotope having an even neutron number, wherein said isotope constitutes not less than 50% of the total mercury content in the spectral lamp. Moreover, the discharge cavity of the spectral lamp is connected to a ballast cavity, the volume of which is greater than the discharge cavity volume, and electrical discharge generating means, the discharge cavity and the ballast cavity are designed in such a way that the discharge generated in the discharge cavity does not penetrates into the ballast cavity. A buffer gas in the spectral lamp contains a noble gas in a quantity equal to or greater than 50%, the charge of the atomic core of said gas being not less than 36. Said invention makes it possible to lower the detection level of mercury in a carrier gas and to reduce a drift.
Abstract: A device for electrophoretic analysis of multicomponent solutions comprises a capillary and vials for electrolyte and sample solutions. The device comprises streaming potential measurement means implemented to measure a potential difference between capillary ends and to form an electric connections with said capillary ends during capillary rinsing in such a way that said streaming potential measurement means and electrolyte inside capillary and vials form a closed measurement electric circuit. A means for generation of electrolyte flow is implemented so as to build up and maintain a preset differential pressure between capillary ends and wherein that streaming potential measurement means comprises means to disconnect said measurement electric circuit during electrophoretic analysis.