Abstract: The invention relates to a device and method for in situ temperature-induced antigen retrieval of samples wherein all steps are performed under a pressure higher than the atmospheric pressure on a sample immobilized on a sample support which can be further subjected to staining and imaging on the same sample support, optionally by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner.

Abstract: Microfluidic cartridge (10) comprising a sampling device (30) having a sealing ring (32) arranged to form a microfluidic chamber (31) when a support containing a biological sample is brought into contact with the sealing ring, and a microfluidic network device (13) configured to supply reagents to the microfluidic chamber. The sampling device further comprises inlet and outlet distribution networks (33a, 33b) in fluid communication with the microfluidic chamber and a slide holder (35) to guide and position said support containing a biological sample on the sampling device. The microfluidic network device comprises a plurality of reagent inlet channels (18) fluidly connectable to reagent sources, at least one reagent outlet channel (22) fluidly connected to the sampling device inlet distribution network (33a), and a plurality of valves (25) operable to selectively connect the inlet channels to the at least one outlet channel.

Abstract: The invention relates to a device and method for in situ temperature-induced antigen retrieval of samples wherein all steps are performed under a pressure higher than the atmospheric pressure on a sample immobilized on a sample support which can be further subjected to staining and imaging on the same sample support, optionally by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner.

Abstract: The invention relates to a method for in situ imaging of samples by cycle multiplexing that enables imaging of various molecular targets through multi-molecular read-outs on the same sample in a rapid, highly sensitive and reliable manner. The invention is further related to imaging buffers preventing the degradation of the sample and of the imaging reagents, which are particularly useful in a method of in situ imaging of samples by cycle multiplexing according to the invention.