Abstract: The subject of the invention is a microscope for high-resolution and specific analysis of biological substances. The microscope according to the invention is characterized by a power supply unit capable of generating constant current attached to the microscope housing; a set of mirrors and illuminating optics; a collimator placed between a monochromatic light source emitting light in a very narrow range of wavelengths and the collector lens; a dark-field condenser and objective including an in-between space to accommodate the stained biological specimen; and a surveillance camera or eyepiece, and a removable color filter placed between the objective and the surveillance camera or the eyepiece. The invention also covers the method to analyze biological specimens with fluorescent staining, wherein the biological contents of the specimen are analyzed in a growing medium in liquid state after the addition of antibodies marked with fluorescent stain, using the microscope according to the invention.

Abstract: This invention relates to a method for the direct detection of tick-borne infections, wherein the human/animal body fluid sample, ideally blood sample to be examined is obtained directly on a specified amount of cell technology medium establishing a partially hostile (semi-hostile) environment, the sample and the cell technology medium are mixed, incubated and stored, if required, meanwhile, the cells and pathogens of the subject are in the semi-hostile environment, then corpuscles and pathogens are separated; a small amount of blood cells from the “buffy coat” layer and blood cells located a few cell layers beneath it are supplemented to and mixed with the gained plasma, the sample is concentrated and the test is finally performed to detect pathogens in the plasma and in blood cells. The claimed matter covers cell technology medium applied and its application interesting methods.