Abstract: An in-line SCSI bus isolation circuit includes two ports for transparently communicating bidirectional data and controls signals therebetween during normal operation using respective dedicated sets of line drivers which may be data bit-by-bit and control signal-by-signal selectively enabled and disabled to permit the bidirectional transparent SCSI communications, and which may be completely disabled to electrically isolate a connected SCSI device on a first SCSI port from the other remaining connected SCSI components on the second SCSI port, so that that device may be removed or inserted onto the SCSI bus at the first port when the line drivers are disabled to prevent the voltage transient signals generated on the first SCSI port from being propagated to the second SCSI port connected to the other SCSI components prior to enabling the line drivers and bidirectional SCSI communications between the two SCSI ports.