Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of disease, for example, breast cancer. A subject can be screened for breast cancer based on altered expression of one or more mRNAs in blood, plasma or salvia. Embodiments include 26 specific mRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with breast cancer. Levels of more than one of the mRNAs can be used to create a biomarker fingerprint for early screening of a breast cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with breast cancer.