Patents Assigned to Marukin Shoyu Co., Ltd.
  • Epimerase
    Patent number: 5994105
    Abstract: A novel polypeptide, acylglucosamine 2-epimerase as shown in FIG. 1 and derivatives thereof, DNA coding for said enzyme, a recombinant vector containing said enzyme, a transformant integrated thereinto said vector, a method for producing said enzyme and a method for producing N-acetylmannosamine and N-acetylneuraminic acid using renin binding protein.
    Type: Grant
    Filed: January 13, 1998
    Date of Patent: November 30, 1999
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta, Isafumi Maru
  • Method for assaying sulfate-conjugated bile acid and therefore
    Patent number: 5919644
    Abstract: In a method of quantifying sulfate-conjugated bile acid in a sample with bile acid sulfate sulfatase and a reductive system indicator, an 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt is used as a reductive system indicator. Sulfate-conjugated bile acid can be quantified through a single reaction without complication.
    Type: Grant
    Filed: August 21, 1997
    Date of Patent: July 6, 1999
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Kenichi Adachi, Yasuhiko Tazuke, Yoji Tsukada
  • Epimerase
    Patent number: 5795767
    Abstract: A novel polypeptide, acylglucosamine 2-epimerase as shown in FIG. 1 and derivatives thereof, DNA coding for said enzyme, a recombinant vector containing said enzyme, a transformant integrated thereinto said vector, a method for producing said enzyme and a mrthod for producing N-acetylmannosamine and N-acetylneuraminic acid using renin binding protein.
    Type: Grant
    Filed: November 22, 1995
    Date of Patent: August 18, 1998
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta, Isafumi Maru
  • Method for preparing N-acetylneuraminic acid
    Patent number: 5665574
    Abstract: A method for preparing N-acetylneuraminic acid characterized in reacting N-acetylglucosamine with pyruvic acid in an alkaline condition by the action of N-acetylneuraminic acid lyase.
    Type: Grant
    Filed: September 14, 1995
    Date of Patent: September 9, 1997
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta
  • Sulfate of n-acetylneuraminic acid homopolymer and processes for making and using the same
    Patent number: 5637690
    Abstract: A sulfate ester of N-acetylneuraminic acid homopolymer represented by the formula (I) or a pharmaceutically acceptable salt thereof: ##STR1## wherein R represents H or SO.sub.3 H, n is 5-1,000, wherein the number of SO.sub.3 H residues per 1 molecule of N-acetylneuraminic acid residues is 0.1 to 3.0, which sulfate ester demonstrates anti-HIV activity.
    Type: Grant
    Filed: December 27, 1994
    Date of Patent: June 10, 1997
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Jang-ho Lee, Yasuhiro Ota, Yoji Tsukada
  • Bile acid sulfate sulfatase gene, plasmid containing said gene and method of producing bile acid sulfate sulfatase
    Patent number: 5585255
    Abstract: The present invention relates to a bile acid sulfate sulfatase gene coding for the amino acid sequence as shown in FIG. 4 and the gene derived from bacteria which belongs to genus Pseudomonas testosteroni, or a bile acid sulfate sulfatase gene as shown in FIG. 3, a plasmid containing the gene, a method for producing a bile acid sulfate sulfatase, and a bile acid sulfate sulfatase.
    Type: Grant
    Filed: October 26, 1993
    Date of Patent: December 17, 1996
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiko Tazuke, Shigenori Okada, Kenichi Adachi
  • Method for preparing N-acetylneuraminic acid by N-acetylneuraminic acid lysase at a pH of 10-12
    Patent number: 5472860
    Abstract: A method for preparing N-acetylneuraminic acid characterized in reacting N-acetylglucosamine with pyruvic acid at an alkaline pH in the presence of N-acetylneuraminic acid lyase.
    Type: Grant
    Filed: September 30, 1993
    Date of Patent: December 5, 1995
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta
  • Process for producing ganglioside G.sub.M1
    Patent number: 5296360
    Abstract: The present invention provides a process for preparing ganglioside G.sub.Ml characterized in that neuraminidase isozyme S is allowed to act on gangliosides to produce ganglioside G.sub.Ml.
    Type: Grant
    Filed: June 28, 1991
    Date of Patent: March 22, 1994
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Process for producing asialo GM1
    Patent number: 5275939
    Abstract: The present invention provides a process for preparing asialo G.sub.M1 characterized in that neuraminidase isozyme L is allowed to act on gangliosides to produce asialo G.sub.M1.
    Type: Grant
    Filed: June 28, 1991
    Date of Patent: January 4, 1994
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Process for preparing neuraminidase
    Patent number: 5116752
    Abstract: The present invention provides a process for preparing neuraminidase comprising incubating a mutant of the genus Arthrobacter capable of producing neuraminidase in the absence of any inducing substance, and recovering neuraminidase from the resulting culture.
    Type: Grant
    Filed: January 15, 1991
    Date of Patent: May 26, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Bile acid sulfate sulfatase, process for its preparation and method for assaying bile acid
    Patent number: 5100795
    Abstract: The invention provides a novel bile acid sulfate sulfatase, a process for its preparation, and a method of assaying bile acid 3.alpha.-sulfates and total bile acids using the bile acid sulfatase.
    Type: Grant
    Filed: April 15, 1991
    Date of Patent: March 31, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiko Tatsuke
  • Bile acid sulfate sulfatase, process for its preparation and method for assaying bile acid
    Patent number: 5091305
    Abstract: The invention provides a novel bile acid sulfate sulfatase, a process for its preparation, and a method of assaying bile acid 3.alpha.-sulfates and total bile acids using the bile acid sulfatase.
    Type: Grant
    Filed: July 26, 1990
    Date of Patent: February 25, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiko Tatsuke
  • Process for producing N-acylneuraminate aldolase
    Patent number: 4699883
    Abstract: This invention provides a process for producing N-acylneuraminate aldolase comprising incubating a mutant of the genus Escherichia capable of producing N-acylneuraminate aldolase even in the absence of any inducing substance, and collecting N-acylneuraminate aldolase from the resulting culture.
    Type: Grant
    Filed: July 23, 1985
    Date of Patent: October 13, 1987
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yoshihiro Uchida, Yasuhiro Ohta