Abstract: Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces and bone are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor.

Abstract: An optical modulating system which allows modulation of a single light beam with a spatially filtering mask to form a spatially periodic, time-dependent excitation field. Once generated, the field can be used to induce a transient grating in a sample. The optical modulating system is additionally configured to automatically orient a probe beam at the Bragg angle, thereby allowing the intensity of the probe beam diffracted off the induced grating to be maximized. Measurement of the diffracted probe beam allows, for example, determination of mechanical, thermal, electrical, and optical properties of the sample.

Abstract: A method for producing semi-permanent casting tooling, including the steps of blending a high char resin with a refractory powder to form a plastic moldable material; shaping the plastic moldable material to form a green tooling body; and heating the green tooling body to convert the high char resin into carbon char to form reusable casting tooling. The casting tooling can be used in casting processes including die casting, permanent mold casting and pressure infiltration casting.

Abstract: An energetic-electron emitter providing electrons having kinetic energies on the order of one thousand electron volts without acceleration through vacuum. An average electric field of 10.sup.5 V/m to 10.sup.10 V/m applied across a layer of emissive cathode material accelerates electrons inside the layer. The cathode material is a high-dielectric strength, rigid-structure, wide-bandgap semiconductors, especially type Ib diamond. A light-emitting device incorporates the energetic-electron emitter as a source of excitation to luminescence.

Abstract: A cathode structure is formed by a process in which a carbon-containing electron-emissive cathode is subjected to electronegative atoms that include oxygen and/or fluorine. The cathode is also subjected to atoms of electropositive metal, typically after being subjected to the atoms of oxygen and/or fluorine. The combination of the electropositive metal atoms and the electronegative atoms enhances the electron emissivity by reducing the work function.

Abstract: An optical communication and/or measurement system includes a transmitter that modulates a pseudo-random noise signal with a message signal to produce a wideband signal for transmission. A receiver, which demodulates the wideband signal to recover the message signal, includes an "analog" feedback shift register ("AFSR") that reproduces the noise signal based on samples of the received signal. The AFSR is a generalization of a linear feedback shift register ("LFSR"). The AFSR is characterized by a function that agrees with the function that characterizes the LFSR, at the points at which that function is defined. The AFSR includes beam splitters that are spaced in accordance with the associated pseudorandom code. The AFSR's function has stable fixed points at integer values and unstable fixed points at half-integer values and, the stable fixed points act as attractors. The AFSR thus produces a sequence that relaxes to the nearest integer-valued sequence.

Abstract: The Wilms' tumor gene associated with 11p3 locus on the human chromosome, as well as a method of analyzing cells for the gene is described and characterized. The gene encodes a transcription unit approximately 50 kb in size and a mRNA of approximately 3 kb, which is expressed in predominantly in kidney and gonadal tissue. The gene is alternative spliced producing four very similar mRNA transcripts. The polypeptides encoded by the Wilms' tumor DNA includes four "zinc fingers" and a region rich in proline and glutamine, suggesting that the polypeptide has a role in transcription regulation.

Abstract: In a "permanent" magnet system based on high-temperature superconductors, a superconducting magnet is cooled to a temperature below its critical temperature with a cooler. The magnet is coupled to a cold body of solid nitrogen which is insulated to minimize heat input to the system. The superconducting magnet is energized to generate a magnetic field and the cooler is removed. Unlike conventional superconducting magnets in which the magnet operating temperature is maintained at a fixed point, in the system of the present invention the operating temperature is allowed to rise from an initial value of approximately 20 K to as high as 63 K (the melting point of nitrogen), enabling operation over an extended period without the aid of direct cooling. The superconducting magnet is recooled periodically to lower its temperature to approximately 20 K. The magnetic field is maintained and the system is fully operable during the recooling process.

Abstract: A computer user interface includes a mechanism for graphically representing and displaying user-definable objects of multiple types. The object types that can be represented include data records, not limited to a particular kind of data, and agents. An agent processes information automatically on behalf of the user. Another mechanism allows a user to define objects, for example by using a template. These two mechanisms act together to allow each object to be displayed to the user and acted upon by the user in a uniform way regardless of type. For example, templates for defining objects allow a specification to be input by a user defining processing that can be performed by an agent.

Abstract: Disclosed is the isolation of a 140,000 kDa protein which binds with high affinity to Z-DNA. Z-DNA is defined herein as a non-B-DNA conformer which is stabilized by negative supercoiling. The isolated protein also has a binding site for double-stranded RNA (dsRNA). Peptide sequences from this protein show similarity to double-stranded RNA adenosine deaminase (dsrad), an enzyme which deaminates adenosine in dsRNA to form inosine. Assays for this enzyme confirm that dsrad activity and Z-DNA binding are properties of the same molecule. The coupling of these two activities in a single molecule indicate a novel mechanism of gene regulation which is in part dependent on DNA topology.

Abstract: A termination device for a flexible tensile strength member has an internal surface which defines a passage through the device. The profile of the internal surface is a constant-radius arc extending from one end of the device to the other end. The radius of the arc emanates from a point of origin located along a line that is both collinear with an end of the device and perpendicular to its longitudinal axis. The design can be optimized either to provide a minimum device outer diameter while generating a high efficiency (greater than 90 percent), or to generate maximum possible efficiency within a device that is minimized in diameter (and wall thickness) while preventing device failure.

Abstract: A light modulator is described which generates an output optical field from an input optical field. The light modulator features first and second polarization-rotating elements which are directly attached to one another. Each element contains an array of pixels including liquid crystals aligned along a specific axis. During operation, the pixels are biased to rotate the liquid crystals. For a particular polarization, the phase and amplitude profiles of the input optical field passing through the pixel array can be arbitrarily and independently controlled across an aperture of the light modulator.

Abstract: A method for preparation of biodegradable polymeric drug delivery devices using relatively low temperatures and non-aqueous solutions which is particularly useful with polyanhydrides, thermolabile drugs, and in forming multi-layered devices.In a first embodiment, the polymer is dissolved in a volatile organic solvent, the drug is dispersed or dissolved in the polymer solution, the mixture is suspended in an organic oil, and the organic solvent is extracted into the oil, creating microspheres. The preferred polymers are polyanhydrides since they are biodegradable and have been proven to be useful in vivo.In a second embodiment, the polymer is dissolved in organic solvent with or without the drug, and the mixture is suspended in glycerol. The suspension is frozen and the organic solvent slowly evaporated.

Abstract: The present invention relates to a method of skin regeneration of a wound or burn in an animal or human. This method comprises the steps of initially covering the wound with a collagen glycosaminoglycan matrix, allowing infiltration of the grafted GC matrix by mesenchymal cells and blood vessels from healthy underlying tissue and applying a cultured epithelial autograft sheet grown from epidermal cells taken from the animal or human at a wound free site on the animal's or human's body surface. The resulting graft has excellent take rates and has the appearance, growth, maturation and differentiation of normal skin.

Abstract: An object movement estimator (400) predicts optical flow by estimating changes in sensed images using one-dimensional correlations. Sensed image portions are stored at a first instant of time and compared with sensed image portions at a second instant of time. An optimal flow is estimated by performing a one-dimensional correlation of the sensed image portions at the first and second instants of time, and a time-to-contact is estimated based on the predicted optical flow thus obtained.

Abstract: Methods and compositions are described herein for reconstruction or augmentation of breast tissue. Dissociated cells, preferably muscle cells, are implanted in combination with a suitable biodegradable, polymeric matrix to form new tissue. There are two forms of matrices which can be used: a polymeric hydrogel formed of a material such as alginate having cells suspended therein, and a fibrous matrix having an interstitial spacing between about 100 and 300 microns. Preferred polymeric materials are those degrading over about one to two months, such as polylactic acid-glycolic acid copolymers. The matrices can be seeded prior to implantation or implanted, allowed to vascularize, then seeded with cells. In a preferred embodiment, the cell-matrix structures are implanted in combination with tissue expander devices. As cell-matrix is implanted, or cells proliferate and form new tissue, the expander size is decreased, until it can be removed and the desired reconstruction or augmentation is obtained.

Abstract: A multi-processing transient event detector for use in a nonintrusive electrical load monitoring system has been provided. Templates of transient pattern data associated with each electrical load which may be monitored are stored. Such templates are then used to match and correlate with the actual monitored transient pattern data at the load site. The decomposition in which time scales are changed is accomplished in parallel along with one or more parallel event detection processing modules for each decomposition.

Abstract: Improved field-emission devices are based on composing the back contact to the emitter material such that electron-injection efficiency into the emitter material is enhanced. Alteration of the emitter material structure near the contact or geometric field enhancement due to contact morphology gives rise to the improved injection efficiency. The devices are able to emit electrons at high current density and lower applied potential differences and temperatures than previously achieved. Wide-bandgap emitter materials without shallow donors benefit from this approach. The emission characteristics of diamond substitutionally doped with nitrogen, having a favorable emitter/vacuum band structure but being limited by the efficiency of electron injection into it, show especial improvement in the context of the invention.

Abstract: The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.

Abstract: A biochemical procedure for identification and characterization of cells in a biopsy or sample of a body fluid. The method can be used to determine cell type, i.e. epidermal, neuronal; tissue of origin, i.e. breast tissue, liver tissue; and degree of abnormality. The procedure can also be used to make antibodies and hybridization probes to detect cell or tissue specific antigens and nuclear matrix associated nucleic acids in cellular material and body fluids.The procedure is based on the isolation and analysis of the components of a specific subcellular protein fraction referred to here as the "nuclear matrix". The nuclear matrix includes proteins and nuclear matrix associated DNA specific to different cell types. These proteins and nucleic acids are altered or new ones expressed as a result of viral infection, genetic defects or malignancy.