Abstract: The present invention provides a combination of genomic and computational technologies to provide rapid, portable sample analysis for sequencing or identifying a target sequence involving generating probes for use in analyzing a sample which may comprise a target sequence.

Abstract: It has been discovered that lung tumor growth is associated with a dysregulation of the local microbiota, including an increased total bacterial load and reduced bacterial diversity in the airway. In the lungs, commensal bacteria, which are otherwise non-pathogenic and colonize pulmonary tissue at a much lower density in healthy individuals, provoke chronic inflammation and exacerbation of lung cancer through tumor-infiltrating immune cells. Thus, targeting the lung microbiota and its responding immune pathways is useful in treating lung cancer. Disclosed are compositions and methods targeting the lung microbiota and its responding immune pathways in a subject by specific targeting of commensal bacteria in the subject. Typically, the methods involve administering an effective amount of one or more therapeutics such as an antibiotic that reduces the local bacterial load, blocks or depletes tumor-infiltrating immune cells, and/or locally inhibits one or more cytokines or chemokines.

Abstract: Chemical reaction devices involving acid and/or base, and related systems and methods, are generally described.

Abstract: Articles, systems, and methods for rapid administration and active pharmaceutical compositions to subjects exposed to radiation and/or toxins are provided by this disclosure. There are currently few interventional technologies to protect against long-term morbidity and mortality from exposure to radiation and chemical warfare. Furthermore, as commercially available technologies such as additive manufacturing and small-scale chemical reactors have become more prevalent, the risk of misuse of such technologies by terrorists and rogue nation states to manufacture nuclear, radiologic, and chemical weapons continues to increase. Articles, systems, and methods described herein combine sensing technologies with drug release components, in order to provide an approach for rapid diagnosis of and response to exposure to radiation and toxic agents.

Abstract: The present disclosure provides patterned biomaterials having organized cords and extracellular matrix embedded in a 3D scaffold. According, the present disclosure provides compositions and applications for patterned biomaterials. Pre-patterning of these biomaterials can lead to enhanced integration of these materials into host organisms, providing a strategy for enhancing the viability of engineered tissues by promoting vascularization.

Abstract: An active three-terminal superconducting device having an intersection region at which a hot spot may be controllably formed is described. The intersection region may exhibit current crowding in response to imbalances in current densities applied to channels connected to intersection region. The current crowding may form a hot spot, in which the superconducting device may exhibit a measurable resistance. In some cases, a three-terminal superconducting device may be configured to sense an amount of superconducting current flowing in a channel or loop without having to perturb the superconducting state or amount of current flowing in the channel. A three-terminal superconducting device may be used to read out a number of fluxons stored in a superconducting memory element.

Abstract: The present invention relates to a cell based genomic Recorded Accumulative Memory (geRAM) system (also referred to herein as Genomically Encoded Memory (GEM)) for recoding data (i.e., changes in nucleic acid sequences in cellular DNA in response to physical and/or chemical signal(s)) from the cellular environment.

Abstract: Systems, devices, and methods for additive manufacturing as disclosed allow for improved optical access to a build platform. In at least some embodiments a multiplexing optic of an additive manufacturing device is configured to multiplex an arbitrary number of optical paths to a build platform along a substantially common optical axis by dividing a theoretical input aperture of the multiplexing optic into a plurality of sub-apertures. Each sub-aperture can independently receive and direct an optical path to the build platform. An optical path can be a light path from a light source or an optical process monitoring path from an optical process monitoring system or optical process monitoring device. In some embodiments, an optical path can enter the multiplexing optic off-axis and/or off-angle with respect to an optical axis of the multiplexing optic. The multiplexing optic can include one or more lens elements and/or one or more mirror elements.

Abstract: The invention, in some aspects, relates to polypeptide molecules and their encoding nucleic acid molecules and use of such molecules to target opsins to the soma of cells in which they are expressed. Compositions of the invention may be delivered to cells and subjects and used in methods to modulate electrical activity of cells in which they are expressed, and for treatment of diseases and conditions in subjects.

Abstract: A prosthesis, including a prosthetic foot, a prosthetic talocrural joint, a prosthetic ankle pivotally coupled to the prosthetic foot and a four-area bending beam. The four-area bending beam includes a U-shaped or other spring. One end of the spring is mechanically coupled via two of the four areas to the prosthetic foot. The other end of the spring is mechanically coupled via the other two areas to the prosthetic ankle. The spring resiliently resists pivoting of the prosthetic ankle about the prosthetic talocrural joint. The four-area bending beam exhibits an at least approximately constant moment along its length to maximize strain energy density, storing about four times as much elastic energy as a comparable cantilevered beam, thereby providing high stiffness and high range of motion to the ankle. The spring is modular, being easily replaced with another spring exhibiting a different stiffness, ex., to tailor the prosthesis to a user.

Abstract: Chemical-shift nuclear magnetic resonance (NMR) spectroscopy involves measuring the effects of chemical bonds in a sample on the resonance frequencies of nuclear spins in the sample. Applying a magnetic field to the sample causes the sample nuclei to emit alternating current magnetic fields that can be detected with color centers, which can act as very sensitive magnetometers. Cryogenically cooling the sample increases the sample's polarization, which in turn enhances the NMR signal strength, making it possible to detect net nuclear spins for very small samples. Flash-heating the sample or subjecting it to a magic-angle-spinning magnetic field (instead of a static magnetic field) eliminates built-in magnetic field inhomogeneities, improving measurement sensitivity without degrading the sample polarization.

Abstract: A heavy metal capture composition, devices including the composition, and a method of reducing heavy metal contamination in the environment is described.

Abstract: Compositions and methods of making compositions for additive manufacturing of composite powders including metal ceramic alloyed material is described.

Abstract: An all-to-all coupled, high-fidelity, error-correctable quantum computer can scale to hundreds of qubits within a single cavity of moderate cooperativity with existing neutral atom technology. This quantum processor can enact teleported gates among any pair of qubits using a local Rydberg interaction between each qubit and a separate network of atoms that distribute entanglement via a cavity mode. Small atomic ensembles at network nodes allow for ultrafast, nondestructive readout with high fidelity by substantially shifting the resonance of even a poor-quality cavity. Fast readout enables near-deterministic entanglement distribution among network atoms despite cavity losses as well as syndrome measurements of qubit atoms for error correction.

Abstract: Systems and methods for flowing particles, such as biological entities, in a fluidic channel(s) are generally provided. In some cases, the systems described herein are designed such that a single particle may be isolated from a plurality of particles and flowed into a fluidic channel (e.g., a microfluidic channel) and/or collected e.g., on fluidically isolated surfaces. For example, the single particle may be present in a plurality of particles of relatively high density and the single particle is flowed into a fluidic channel, such that it is separated from the plurality of particles. The particles may be spaced within a fluidic channel so that individual particles may be measured/observed over time. In certain embodiments, the particle may be a biological entity. Such article and methods may be useful, for example, for isolating single cells into individual wells of multi-well cell culture dishes (e.g., for single-cell analysis).

Abstract: Disclosed herein are systems and methods of obtaining a derivative Raman spectrum using an excitation or Raman pump beam whose wavelength is modulated in any suitable manner such as, for example, stochastically. Shifting the wavelength of the input excitation by a small amount in approaches like SERDS can isolate the Raman scatter from other spectral artifacts and reduce the false detection rate. For example, an input excitation sequence can be correlated with the response of an individual pixel of a detector. From this, pixels that have captured Raman scattered photons can be separated from pixels capturing non-Raman photons. These techniques can be expanded to other fields and/or types of spectroscopies that utilize a dispersive element detector with time-dependent spectral features.

Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

Abstract: System, methods, and other embodiments described herein relate to improving the estimation of battery life. In one embodiment, a method includes measuring electrochemical data of a battery cell associated with an electrochemical reaction triggered by a test during a diagnostic cycle. The method also includes determining a feature associated with the degradation of the battery cell from the electrochemical data. The method also includes predicting an end-of-life (EOL) of the battery cell by using the feature in a machine learning (ML) model.

Abstract: A neuromodulator may output stimuli that causes a user to fall asleep faster than the user would in the absence of the stimuli. Alternatively, the stimuli may modify a sleep state or behavior associated with a sleep state, or may cause or hinder a transition from a waking state to a sleep state or from a sleep state to another sleep state. The neuromodulator may take electroencephalography measurements. Based on these measurements, the neuromodulator may detect, in real time, instantaneous amplitude and instantaneous phase of an endogenous brain signal. The neuromodulator may output stimulation that is, or that causes sensations which are, phase-locked with the endogenous brain signal. In the course of calculating instantaneous phase and amplitude, the neuromodulator may perform an endpoint-corrected Hilbert transform. The stimuli may comprise auditory, visual, electrical, magnetic, vibrotactile or haptic stimuli.

Abstract: A platform technology has been designed to provide a means for controlled delivery of single or multiple doses of therapeutic, prophylactic, diagnostic or identifying agents to livestock. The delivery system is based on a livestock ear tag that releases therapeutic and/or prophylactic agent when applied to the ear or other desired anatomical target of the animal. The agent to be delivered is encapsulated in or on microneedles and or microparticles and or nanoparticles or combination thereof on a surface thereon of the male or female part of the tag, which is pressed into the skin so that the microneedles penetrate into the epidermis and dermis layers of the skin. The agent is then released into the animal from the microneedles and or microparticles and or nanoparticles or combination thereof at the site of contact into the epidermis and dermis layers of the skin.