Abstract: Methods are provided for production of differentiated tissue equivalent model systems from cryopreserved undifferentiated cells. The methods include seeding undifferentiated cells directly onto a support for differentiation immediately after recovery from cryopreservation, without intervening steps for expansion, harvesting, counting, or dilution of the cells.
Type:
Application
Filed:
October 6, 2016
Publication date:
April 12, 2018
Applicant:
MatTek Corporation
Inventors:
Patrick J. Hayden, Michael Bachelor, Mitchell Klausner
Abstract: Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent.
Type:
Application
Filed:
June 24, 2005
Publication date:
January 3, 2008
Applicant:
MatTek Corporation
Inventors:
Mitchell Klausner, Seyoum Ayehunie, Joseph Kubilus
Abstract: Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent.
Type:
Grant
Filed:
June 7, 2002
Date of Patent:
September 13, 2005
Assignee:
MatTek Corporation
Inventors:
Mitchell Klausner, Seyoum Ayehunie, Joseph Kubilus
Abstract: Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent.
Type:
Application
Filed:
June 7, 2002
Publication date:
December 11, 2003
Applicant:
MatTek Corporation
Inventors:
Mitchell Klausner, Seyoum Ayehunie, Joseph Kubilus