Abstract: The invention is directed to a method for creating an optical tomogram, which comprises the steps providing an optical microscope, arranging a sample (1) in the optical coverage region of a lens (5) of the microscope, setting the focus of the lens to a particular focal plane (2), recording an image of the sample through the microscope, rotating the sample through an angle ?, optionally displacing the sample along the longitudinal axis (z) of the lens (5) and/or perpendicular to the plane of the previously recorded image (9) and continuing the method with step d) until a predetermined number of section images (9) of the sample (1) have been recorded, wherein the sample (1) is displaced along the longitudinal axis (z) of the lens (5) and/or perpendicular to the plane of the previously recorded image (9), in accordance with step f), at least once during a rotation of the sample through 360°.