Abstract: An apparatus and methods for electroporation of cells are disclosed. The apparatus comprises a memory storing instructions, a high voltage module to apply a voltage to cells, a low voltage module including a user interface, a system board connected to the high voltage module, the low voltage module, and a single board computer, and the single board computer including at least one processor to control the high voltage module and the low voltage module. The system board includes an emergency stop button and an electronic circuit. The electronic circuit receives a signal from the emergency stop button, latches the signal from the emergency stop button, blocks a logical signal controlling the voltage delivered to the instrument output, terminates the voltage applied to cells, and disables the at least one processor to provide commands to the high voltage module.

Abstract: Method of electroporating with cell culture aimed to improve cell viability wherein the method comprises electroporating cells of interest with an anti-apoptosis protein, treating the cells with DNase post-electroporating, and shifting the temperature of the cells from 37° C. to 32° C. while resting post electroporation.

Abstract: Method of cell-editing comprising combining a cell or cell line with a virus, viral vector or virus like particle to form a mixture and performing simultaneous electroporation and transduction on the mixture to insert therein the virus, viral vector or virus like particle. The disclosed method simultaneously causes the virus, viral vector or virus like particle to edit, remove or modify a cell or cell line and inserting a virus, viral vector or virus like particle therein. A modified cell or cell line made by the disclosed method is also disclosed.

Abstract: Aspects of the disclosure are directed to a technique for sequential electroporation that provides for a delivery of multiple electrical pulses separated in time to cells, cell particles, lipid vesicles, liposomes, or to increase efficiency of entry of one or more agents of interest into cells, cell particles, lipid vesicles, liposomes, tissues, or derivatives thereof, and to minimize damage by electrical arc or heat shock; increase loading efficiency of an agent of interest; and maintain viability of the cells, cell particles, lipid vesicles, or tissues and the ability of the cells, cell particles, lipid vesicles, liposomes, or tissues to produce a clinical effect.

Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: contacting the cells with an activating composition; transfecting the cells with a transfection composition comprising (a) donor DNA and (b) a DNA digesting agent; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region; and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.