Patents Assigned to Maxygen, Inc.
  • Methods and compositions for polypeptide engineering
    Patent number: 6653072
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: June 24, 1999
    Date of Patent: November 25, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • DNA shuffling of monooxygenase genes for production of industrial chemicals
    Publication number: 20030215859
    Abstract: This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as &agr;-hydroxycarboxylic acids, and aryl- and alkyl-hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectivity, enzymatic activity, stereospecificity, and the like. Methods for obtaining recombinant polynucleotides that encode these improved polypeptides are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses. Also provided by the invention are methods for increasing said solvent resistance of organisms that are used in the synthetic methods.
    Type: Application
    Filed: April 4, 2003
    Publication date: November 20, 2003
    Applicant: MAXYGEN, INC.
    Inventors: Joseph A. Affholter, S. Christopher Davis, Sergey A. Selifonov
  • Optimization of crossover points for directed evolution
    Publication number: 20030198988
    Abstract: Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.
    Type: Application
    Filed: March 10, 2003
    Publication date: October 23, 2003
    Applicant: Maxygen, Inc.
    Inventors: Emily C. Mundorff, Sridhar Govindarajan, Claes Gustafsson, Jeremy S. Minshull
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Publication number: 20030186356
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Application
    Filed: September 17, 2002
    Publication date: October 2, 2003
    Applicant: Maxygen, Inc.
    Inventor: Willem P.C. Stemmer
  • Methods and compositions for polypeptide engineering
    Patent number: 6613514
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: April 27, 2000
    Date of Patent: September 2, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • DNA shuffling of monooxygenase genes for production of industrial chemicals
    Patent number: 6605430
    Abstract: This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as &agr;-hydroxycarboxylic acids, and aryl- and alkyl-hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectivity, enzymatic activity, stereospecificity, and the like. Methods for obtaining recombinant polynucleotides that encode these improved polypeptides are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses. Also provided by the invention are methods for increasing said solvent resistance of organisms that are used in the synthetic methods.
    Type: Grant
    Filed: August 12, 1999
    Date of Patent: August 12, 2003
    Assignee: Maxygen, Inc.
    Inventors: Joseph A. Affholter, S. Christopher Davis, Sergey A. Selifonov
  • Evolution of whole cells and organisms by recursive sequence recombination
    Publication number: 20030148309
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites
    Type: Application
    Filed: July 11, 2002
    Publication date: August 7, 2003
    Applicant: Maxygen, Inc.
    Inventors: Stephen del Cardayre, Matthew Tobin, Willem P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda A. Castle, Claus M. Krebber, Steven Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph A. Affholter
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6602986
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: November 15, 2000
    Date of Patent: August 5, 2003
    Assignee: Maxygen, Inc.
    Inventors: Willem P. C. Stemmer, Andreas Crameri
  • Novel co-stimulatory molecules
    Publication number: 20030138881
    Abstract: The invention provides polynucleotides and polypeptides encoded therefrom having advantageous properties, including an ability of the polypeptides to preferentially bind a CD28 or CTLA-4 receptor at a level greater or less than the ability of human B7-1 to bind CD28 or CTLA-4, or to induce or inhibit altered level of T cell proliferation response greater compared to that generated by human B7-1. The polypeptides and polynucleotides of the invention are useful in therapeutic and prophylactic treatment methods, gene therapy applications, and vaccines.
    Type: Application
    Filed: December 20, 2001
    Publication date: July 24, 2003
    Applicant: Maxygen, Inc.
    Inventors: Juha Punnonen, Alexandra Lazetic, Steven R. Leong, Chia-Chun Chang, Doris Apt, Claes Gustafsson
  • Modification of virus tropism and host range by viral genome shuffling
    Patent number: 6596539
    Abstract: The invention relates to a method and compositions for modifying a phenotype of a virus, such as viral tropism and host range, by iterative sequence recombination of variant viruses and selection of improved variants.
    Type: Grant
    Filed: October 30, 1998
    Date of Patent: July 22, 2003
    Assignee: Maxygen, Inc.
    Inventors: Willem P. C. Stemmer, Phillip Patten, Nay Wei Soong
  • Modification of virus tropism and host range by viral genome shuffling
    Publication number: 20030124708
    Abstract: The invention relates to a method and compositions for modifying a phenotype of a virus, such as viral tropism and host range, by iterative sequence recombination of variant viruses and selection of improved variants.
    Type: Application
    Filed: December 4, 2002
    Publication date: July 3, 2003
    Applicant: Maxygen, Inc.
    Inventors: Willem P.C. Stemmer, Phillip Patten, Nay Wei Soong
  • Methods and compositions for polypeptide engineering
    Patent number: 6586182
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: October 20, 2000
    Date of Patent: July 1, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Methods and compositions for polypeptide engineering
    Patent number: 6579678
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: October 20, 2000
    Date of Patent: June 17, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Methods for producing recombined antibodies
    Patent number: 6576467
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: November 21, 2000
    Date of Patent: June 10, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Polynucleotides encoding flavivirus and alphavirus multivalent antigenic polypeptides
    Patent number: 6576757
    Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: June 10, 2003
    Assignee: Maxygen, Inc.
    Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
  • Nucleic acid libraries
    Patent number: 6573098
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: June 3, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Flavivirus and alphavirus recombinant antigen libraries
    Patent number: 6569435
    Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: May 27, 2003
    Assignee: Maxygen, Inc.
    Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
  • Lipid acyl hydrolases and variants thereof
    Publication number: 20030097684
    Abstract: The present invention provides compositions comprising lipid acyl hydrolases with improved enzymatic activity and methods for using such compositions to enhance resistance of plants to pests.
    Type: Application
    Filed: December 27, 2001
    Publication date: May 22, 2003
    Applicant: Maxygen, Inc.
    Inventors: Brian Carr, Barbara A. Rosen, Ericka R. Bermudez, Jon E. Ness
  • Novel glyphosate N-acetyl transferase (GAT) genes
    Publication number: 20030083480
    Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structrurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.
    Type: Application
    Filed: October 29, 2001
    Publication date: May 1, 2003
    Applicant: Maxygen, Inc.
    Inventors: Linda A. Castle, Dan Siehl, Lorraine J. Giver, Jeremy Minshull, Cristina Ivy, Yong Hong Chen, Nicholas B. Duck
  • Combinatorial libraries of monomer domains
    Publication number: 20030082630
    Abstract: Methods for identifying discrete monomer domains and immuno-domains with a desired property are provided. Methods for generating multimers from two or more selected discrete monomer domains are also provided, along with methods for identifying multimers possessing a desired property. Presentation systems are also provided which present the discrete monomer and/or immuno-domains, selected monomer and/or immuno-domains, multimers and/or selected multimers to allow their selection. Compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.
    Type: Application
    Filed: April 26, 2002
    Publication date: May 1, 2003
    Applicant: Maxygen, Inc.
    Inventors: Joost A. Kolkman, Willem P.C. Stemmer