Abstract: There is provided a method and use of hematopoietic stem cells for the treatment of various diseases. The differentiation of autologueous hematopoietic stem cells into myeloid lineages is used for reintroduction into a patient in addition to undifferentiated stem cells for the purpose of reconstituting both the defence and long-term engraftment.

Abstract: There is provided co-culture bioreactor systems that can maintain stem cells and differentiated cell types in physically isolated environments but can allow biochemical communication between these cells. For instance, a co-culture bioreactor system of the present invention can include a first culture chamber that defines a first inlet and a first outlet such that fluid can flow through the culture chamber. The system can also include a second culture chamber defining a second inlet and a second outlet allowing a second fluid flow through this second chamber. The system can also include a semi-permeable membrane. The semi-permeable membrane can be located between the first culture chamber and the second culture chamber.