Abstract: A novel method for determining the levels of TGF-?1 or TGF-?2 in a sample of milk, raw protein source, or nutritional composition is provided. The method involves, in some cases, reconstituting the sample; in some cases, centrifuging the sample; activating the sample using particular ratios of sample:acid:base; diluting the sample using particular ratios of sample:buffer agent; and determining the concentration of TGF-?1 in the sample using an ELISA assay.
Type:
Application
Filed:
February 12, 2009
Publication date:
April 29, 2010
Applicant:
MEAD JOHNSON & CO.
Inventors:
Gyan P. Rai, Francisco J. Rosales, Zeina E. Jouni, Rosaline Waworuntu
Abstract: The present invention is directed to a processing method and a selection of ingredients that will retain TGF-? levels, bioactivity, and/or bioavailability in a liquid nutritional product.
Type:
Application
Filed:
February 12, 2009
Publication date:
April 29, 2010
Applicant:
Mead Johnson & Co.
Inventors:
Juan M. Gonzalez, Dattatreya Banavara, John A. Alvey
Abstract: The present invention is directed an improved nutritional composition, methods of improving digestion, and methods of enhancing the bioavailability of TGF-?.
Type:
Application
Filed:
February 13, 2009
Publication date:
April 29, 2010
Applicant:
Mead Johnson & Co.
Inventors:
Dattatreya Banavara, Win-Chin Chiang, Deborah A. Schade, Joaquin A. Franco, Juan M. Gonzalez, Dirk Hondmann, Zeina E. Jouni, Yung-Hsiung Lee, Robert J. McMahon, Kristin Morris, Deshanie Rai, Gyan P. Rai, Nagendra Rangavajla, Francisco J. Rosales, William Michael Russell, Eric Van Tol, Hugh N. Tucker, Donald Carey Walker
Abstract: A novel method for determining the bioactivity of TGF-? in a sample of milk, raw protein source, or nutritional composition is provided. The method includes particular reconstitution steps, centrifugation steps, incubation steps, and activation steps. The bioactivity of the TGF-? in the sample may be measured in a HT-2 cell bioassay or a cellomics bioassay.
Type:
Application
Filed:
February 13, 2009
Publication date:
April 29, 2010
Applicant:
MEAD JOHNSON & CO.
Inventors:
Gyan P. Rai, Francisco J. Rosales, Zeina E. Jouni, Rosaline Waworuntu
Abstract: The present invention is directed to a novel method for increasing the production of acetate, decreasing the production of butyrate, increasing the population and species of beneficial bacteria and slowing the rate of fermentation of prebiotics within the gut of a formula-fed infant. The method comprises administration of a therapeutically effective amount of PDX to the infant.
Type:
Application
Filed:
February 12, 2009
Publication date:
July 9, 2009
Applicant:
Mead Johnson & Co.
Inventors:
Bryon W. Petschow, Robert J. McMahon, Glenn R. Gibson, Robert A. Rastall, Renia Gemmell, Maria Saarela, Anna-Marja Aura