Abstract: An arterial closure device (20) comprises a body (26) having first and second ports (28, 30) and a catheter (32) having proximal and distal ends (34, 36) extending from the body (26). The catheter (32) defines first and second lumens (38, 40) in operative communication with the first and second ports (28, 30). A first balloon (50) is positioned adjacent the distal end (36) of the catheter (32) and is operatively coupled to the first lumen (38) to receive a fluid through the first port (28) to expand the first balloon (50). A second balloon (58) is operatively coupled to the second lumen (40) to receive a clotting agent (60) through the second port (30) to inflate the second balloon (58). At least one slit (62) is disposed in the second balloon (58) and the slit (62) is expandable between open and closed positions in response to inflation of the second balloon (58) such that the clotting agent (60) is ejected through the slit (62) in the open position to close the puncture hole (22).