Abstract: A method of obtaining fluorochromes that bind to specific sites on proteins is presented. The method consists of synthesizing a peptide bearing a sequence from a parent protein and a second peptide of similar composition but with a scrambled sequence. Each peptide is then added to a test fluorochrome at similar concentrations, and a difference in fluorescence is obtained. A difference in fluorescence indicates a sequence dependent interaction between the peptide and fluorochrome. A large number of fluorochromes can be screened using the two peptides to find one binding in a sequence dependent fashion. This fluorochrome can then be tested for binding to the original full-length protein. The fluorochrome can then serve as scaffold in medicinal chemistry. The fluorochrome/peptide interaction and resulting fluorescence can be inhibited by non-fluorescent test compounds, which are compounds that bind to this peptide. Thus.