Abstract: The present invention relates to liver lactogenic factor and to its isolation, purification and characterization. The liver lactogenic factor of the present invention is useful to increase mil and/or casein production in lactating female mammals. The invention additionally pertains to a novel bioassay capable of identifying lactogenic factors.

Abstract: A method is described for separating fused cells, resulting from fusion of human cells known to produce a specific antibody or a specific lymphokine with malignant human partner cells, from the said partner cells which comprises addition of specific antiserum capable of identifying antigenic specificities unique to the clone and non-reactive with the non-fused partner cells. After reaction of the fused cell with the antiserum, the reaction product is separated within 24 hours by indirect rosetting.One cell line, ATCC HB-8143, secreted both IgG and IgM monoclonal antibodies, both antibodies having specificity to human breast carcinoma and being highly selective therefor.

Abstract: Described herein are methods for quantifying in vitro various parameters related to the use of transfer factor (e.g., dialyzable leukocyte extract containing transfer factor) in the therapeutic and/or prophylactic treatment of patients having antigen-selective defects in cell mediated immunity, so as to standardize such parameters, predict or estimate therefrom the effectiveness of such treatment in vivo, determine optimum dosages and/or administrative regimens therefor and monitor the progress of patients receiving such treatment.In one embodiment of the invention, the quantification of the various parameters is based upon in vitro assays which measure mediators of cellular immunity produced and released by appropriate leukocytes (e.g., those of the patient before treatment, those of potential donors, those of target cells, those of the patient after treatment with transfer factor, and the like) sensitized to a specific antigen.