Abstract: A method for determining a level of one or more live microbes in a sample of a Cannabis plant includes (a) introducing a DNase enzyme into a sample including (i) Cannabis plant matrix derived from lysed cells of a Cannabis plant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and/or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).

Abstract: The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.

Abstract: The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.

Abstract: Using the efficiency of next generation sequencing, a draft de novo reference sequence for the Cannabis (C.) Sativa and C. Indica genomes has been generated as well as four full length contiguous sequences with homology to THCA and CBDA synthases and 10 partially homologous contigs with truncated ORFs. In particular aspects the invention is directed to an (one or more) isolated sequence (e.g., nucleic acid sequence, DNA, RNA, genomic sequence, polypeptide) of a Cannabis genome and uses thereof.