Abstract: A recombinant DNA expression vector comprising an Epstein-Barr virus origin of replication, an EBNA-1 gene, a selectable marker expression cassette and a human gamma interferon expression cassette comprising a promoter region, an open reading frame encoding human gamma interferon and a polyadenylation region; a mammalian host cell transformed by said vector; and a process for producing human gamma interferon using said transformed mammalian host cell.

Abstract: This invention relates to a method for promoting periodontal regeneration in humans, the method comprising(a) application of an agent which demineralizes enamel and dentin, and subsequently(b) application of an agent which stimulates the formulation of endothelial cells.

Abstract: A method and device for detecting an antigenic material in which the device comprises a test utensil having an indentation in which two reagent spots are placed, the first body being a dyed substrate having a coating of an antibody or antibody-like material thereon and the second of the two reagent spots comprising a dyed test-inert material or a dyed substrate with a coating of a normal animal serum, the dye employed in the second reagent spot having a different color than that employed in the first spot. When a liquid test sample is added to the indentation, the dyed substrate particles or components are suspended or solubilized, and the resulting suspension gives the appearance of a third color. A positive agglutination test is indicated by the formation of at least one spot having the color of the first dyed substrate against a background having the color of the second dyed substrate.

Abstract: Disclosed is a process for the purification of crude immune interferon to a near homogeneous preparation which comprises: (a) adsorbing the crude interferon onto a column containing Controlled Pore Glass beads and eluting with ammonium sulfate, (b) adsorbing the interferon containing eluant onto a column containing either Concanavalin A-Sepharose, lentil lectin-Sepharose or pea lectin-agarose and eluting with a buffer containing a sugar, (c) adsorbing the interferon containing eluant onto a column containing Heparin-Sepharose or Procian Red-agarose and eluting with a high salt content buffer, (d) adsorbing the interferon containing eluant onto a cationic exchange resin column and eluting with a salt buffer and (e) treating the interferon containing eluant in a gel-filtration column equilibrated in high salt to obtain a solution of immune interferon that is nearly homogeneous.

Abstract: Disclosed is a process for the purification of crude immune interferon to a near homogeneous preparation which comprises: (a) adsorbing the crude interferon onto a column containing Controlled Pore Glass beads and eluting with ammonium sulfate, (b) adsorbing the interferon containing eluant onto a column containing either Concanavalin A-Sepharose, lentil lectin-Sepharose or pea lectin-agarose and eluting with a buffer containing a sugar, (c) adsorbing the interferon containing eluant onto a column containing Heparin-Sepharose or Procian Red-agarose and eluting with a high salt content buffer, and (d) treating the interferon containing eluant in a gel-filtration column equilibrated in high salt to obtain a solution of immune interferon that is nearly homogeneous.

Abstract: A process is disclosed for producing human immune interferon with cell cultures of human peripheral blood leukocytes modulated with Mezerein or 12, 13 phorbol dibutyrate and induced with the calcium ionophore A-23187. After cultures are incubated for 12 hours to about 7 days, crude interferon on the order of 10.sup.3 units of interferon/ml. is produced.

Abstract: A process is disclosed for producing human immune interferon with cell cultures of human peripheral blood leukocytes modulated with Mezerein or 12,13 phorbol dibutyrate and induced with Staphylococcal aureus enterotoxin B. After cultures are incubated for 3 to 7 days crude interferon on the order of 10.sup.3 units of interferon/ml. is produced.

Abstract: A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.

Abstract: An improved test for assessing the unsaturated binding capacity of serum proteins which bind thyroid hormones employs a clay-organic complex for adsorbing a free radioactive hormone fraction. The sorbent, an organically modified montmorillonite, provides better separation of thyroid states and may be employed in smaller amounts than prior sorbents employed in comparable tests.

Abstract: Ozone generated for chemiluminescent reaction in an analyzer for the measurement of oxides of nitrogen, for example, is purified by passing the ozone through a bed of silica gel, thereby removing substances that interfere with the analysis.

Abstract: Hydrogen sulfide scrubber for use with sulfur compound analyzers effectively removes hydrogen sulfide from gas streams, such as ambient air, without removing substantial amounts of other sulfur compounds, particularly sulfur dioxide. The scrubber employs a bed of silver sulfate, silver nitrate, or silver iodide and operates effectively over wide temperature and humidity ranges without requiring temperature control, with minimal interference from ozone, and operates at flow rates employed by conventional sulfur analyzers without excessive pressure drops.

Abstract: A catalytic converter comprises a block having a bore receiving a tube coaxially, the tube being suspended in the bore by a fitting threaded into one end of the bore and being spaced from the wall of the bore so that a gas may flow along the exterior of the tube and then through the interior of the tube. For converting nitrogen dioxide to nitric oxide, the tube contains copper granules held in place by plugs of copper turnings. Temperature control and regulation are also provided.

Abstract: Precisely controlled permeation of a fluid into a carrier gas is provided in a temperature-controlled enclosure. A cylindrical housing has its side wall spaced from the side wall of a hollow cylindrical core contained therein to provide a thin flow passage therebetween for the carrier fluid. An electric heater coil is wound about the exterior of the housing to supply heat to the carrier fluid in the passage, and the core has a port at one end thereof for providing communication between the passage and the hollow interior of the core, in which a permeation tube is supported. The heater power output is controlled in response to a temperature sensor within the core so that the carrier fluid is heated and its temperature is precisely controlled. The exterior of the housing is insulated from ambient to maintain stable temperature conditions.

Abstract: A copper catalyst maintained in the temperature range of 220.degree. to 240.degree.C. is employed to convert nitrogen dioxide to nitric oxide, as in the measurement of oxides of nitrogen by chemiluminescent techniques. The converter comprises a body having a bore receiving a copper tube coaxially, the tube being spaced from the wall of the bore so that a gas containing nitrogen dioxide may flow along the exterior of the tube and then through the interior of the tube, which contains copper granules held in place by plugs of copper turnings. The temperature of the body is maintained by heater tape wound about the body, and the body is enclosed within an insulated container.

Abstract: Liquid scintillation detection system employs improved sample holders in which the cap of a glass vial is provided with a well for receiving a standard laboratory test tube containing a radioactive sample. The well is immersed in a liquid scintillator in the vial, the scintillator containing lead acetate solution to enhance its efficiency. A commercially available beta-counting liquid scintillation apparatus is modified to provide gamma-counting with the improved sample holders.