Abstract: A process for the determination of H. pylori in a fecal specimen comprising (a) dispersing a fecal specimen suspected of carrying H. pylori in a sample diluent; (b) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen; (c) separating said specimen and said complex; (d) exposing the complex to a second polyclonal antibody for said antigen and a portion of the antibody reacting with said complex, one of said first and second antibody being bound to a solid carrier and the other being labeled with a detection agent; and (e) determining the amount of the labeled antibody and in turn determining the presence of H. pylori antigen in said fecal specimen.
Type:
Grant
Filed:
March 10, 1998
Date of Patent:
August 3, 1999
Assignee:
Meridian Diagnostics, Inc.
Inventors:
Christopher Vance Larka, Ching Sui Arthur Yi, Kenneth James Kozak
Abstract: A method for staining fecal specimens to diagnose stool parasites preserved with a non-mercury fixative wherein the preserved specimen is stained with a composition of iron hematoxylin stain and trichrome stain.
Type:
Grant
Filed:
March 5, 1996
Date of Patent:
February 23, 1999
Assignee:
Meridian Diagnostics, Inc.
Inventors:
Jeffrey A. Kraft, Ching Sui A. Yi, David Huntington Willis, Jr.
Abstract: A process for the determination of H. pylori in a fecal specimen which comprises; (a) collecting a smear of a fecal specimen on a substrate; (b) immersing at least the portion of the substrate carrying the smear in a sample diluent so as to disperse the fecal specimen in the diluent; (c) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen; (d) separating the specimen and the complex; (e) exposing the complex to a second polyclonal antibody for the antigen and a portion of the antibody reacting with the complex, one of said first and second antibody being bound to a solid carrier and the other being labelled with a detection agent; and (f) determining the amount of the labelled antibody and in turn determining the presence of H. pylori antigen in the fecal specimen.
Type:
Grant
Filed:
July 21, 1997
Date of Patent:
February 16, 1999
Assignee:
Meridian Diagnostics, Inc.
Inventors:
Christopher Vance Larka, Ching Sui Arthur Yi, Kenneth James Kozak
Abstract: A process for the determination of H. pylori in a fecal specimen comprising (a) dispersing a fecal specimen suspected of carrying H. pylori in a sample diluent; (b) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen; (c) separating said specimen and said complex; (d) exposing the complex to a second polyclonal antibody for said antigen and a portion of the antibody reacting with said complex, one of said first and second antibody being bound to a solid carrier and the other being labeled with a detection agent; and (e) determining the amount of the labeled antibody and in turn determining the presence of H. pylori antigen in said fecal specimen.
Type:
Grant
Filed:
May 9, 1996
Date of Patent:
February 10, 1998
Assignee:
Meridian Diagnostics, Inc.
Inventors:
Christopher Vance Larka, Ching Sui Arthur Yi, Kenneth James Kozak