Abstract: The present invention relates to a method for detecting a pathogen in cellular lysate by measuring pathogen-specific enzyme activity. The method comprises contacting the cellular lysate with a substrate the pathogen of interest recognizes and modifies, and obtaining a measurable, recordable, signal. The method may comprise detection of SARS-CoV viruses using the activity of SARS PLpro enzyme in tongue scrape lysate as a readout.

Abstract: The present invention relates to a method for detecting a pathogen in cellular lysate by measuring pathogen-specific enzyme activity. The method comprises contacting the cellular lysate with a substrate the pathogen of interest recognizes and modifies, and obtaining a measurable, recordable, signal. The method may comprise detection of SARS-CoV viruses using the activity of SARS PLpro enzyme in tongue scrape lysate as a readout.

Abstract: A pulse analysis system or method includes a frequency filter that receives an ultrafast pulse under test and disperses the pulse under test over a frequency range. The frequency filter separates the pulse under test into component frequency slices and provides the frequency slices to a detector coupled to a digitizer, which processes the digitized signal and collects a sonogram characteristic of the pulse under test. The frequency slices are arranged to overlap. Ptychography is performed on the sonogram to obtain characteristics of the pulse under test.

Abstract: The present invention relates to a method for detecting a pathogen in cellular lysate by measuring pathogen-specific enzyme activity. The method comprises contacting the cellular lysate with a substrate the pathogen of interest recognizes and modifies, and obtaining a measurable, recordable, signal. The method may comprise detection of SARS-CoV viruses using the activity of SARS PLpro enzyme in tongue scrape lysate as a readout.

Abstract: A pulse analysis system or method includes a frequency filter that receives an ultrafast pulse under test and disperses the pulse under test over a frequency range. The frequency filter separates the pulse under test into component frequency slices and provides the frequency slices to a detector coupled to a digitizer, which processes the digitized signal and collects a sonogram characteristic of the pulse under test. The frequency slices are arranged to overlap. Ptychography is performed on the sonogram to obtain characteristics of the pulse under test.

Abstract: A frequency resolved optical gating (FROG) system receives an ultrafast laser pulse as the “unknown wave” input to the system. The FROG system preferably generates a spectrogram (FROG trace) of the input pulse using a polarization gate, second harmonic generation or other FROG geometry. The system or method preferably analyzes the spectrogram using constrained outer products and principal component generalized projections to find characteristics of the unknown wave such as intensity and phase. Examples of constrained outer products include outer products that incorporate an external constraint such as spectral information or an internal constraint such as a relationship between the probe and gate components derived from the unknown wave.

Abstract: The described system and method uses data from interaction between a known wave and an unknown wave to analyze or characterize the unknown wave using cross correlation frequency resolved optical gating (X-FROG). The system may obtain X-FROG trace data from the interaction between the two waves. The system analyzes the X-FROG trace data using a modified principal component generalized projection method strategy to invert the X-FROG trace data, analyzing or characterizing the unknown wave. Results of the analysis can be provided in real time and displayed.

Abstract: A real-time FROG system provides ultra fast pulse measurement and characterization. The system includes direct, integrated feedback that measures how well the system is retrieving pulses and tracking changes in the pulse train. This feedback is provided in real time and may be in the form of the FROG trace error, the display of the measured and retrieved FROG trace, accuracy of background subtraction or other quality measurement. The system includes preprocessing options that can be used to adjust the dynamic range of the measured signal or to perform different types of filtering. The preprocessing of the FROG trace precedes phase retrieval processing and improves the quality of pulse retrieval.