Abstract: A method for detecting and counting the microorganisms in a sample is described. The method comprises: a) selectively enriching the microorganism sought in the sample, b) inducing or activating at least one enzymatic activity of the microorganism, c) immunomagnetically concentrating the microorganism, d) fluorescently labeling the microorganism, and e) detecting and analyzing the fluorescence making possible the numeration or counting of the microorganisms sought by flow cytometry, filtration cytometry or fluorescence microscopy.
Type:
Grant
Filed:
October 1, 2003
Date of Patent:
November 11, 2014
Assignee:
Metis Biotechnologies
Inventors:
Bruno Vedrine, Aline Lachaise, Vincent Carre