Abstract: A microfluidic device and a microfluidic chip are provided. The microfluidic device includes the microfluidic chip, a pouring element, a flow adjustment element and a processor. The microfluidic chip includes a sorting assembly, a sample outlet channel, a pouring channel, a collection channel and a waste channel. The sorting assembly includes a sample inlet channel and a sorting chamber. The pouring element is connected to the pouring channel. The flow adjustment element is connected to a distal end of the sample outlet channel. The processor is configured to control the pouring element to pour a guiding fluid into the pouring channel entering the sample outlet channel and control the flow adjustment element to adjust a flow resistance of a drain section of the sample outlet channel.

Abstract: In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.

Abstract: The present invention provides a fluidic connector, to combine with a microfluidic chip cartridge, comprising: a base, having a plurality of through holes; and a plurality of soft tubes, penetrating the base via the through holes and fixed on the base, and having a Shore A hardness in a range of 50-99, wherein an end of the plurality of soft tubes is protruded from a first side of the base to form plurality of protrusion portions and each protrusion portion has a length in a range of 0.5-10 mm. The present invention also provides a microfluidic chip cartridge and a fluidic connector assembly.

Abstract: A method for predicting immunotherapy response of a subject having cancer includes the following steps. A peripheral blood sample is obtained from the subject having cancer before or after receiving the immunotherapy. The number of immune cells in the peripheral blood sample of the subject having cancer is detected. The number of immune cells and a first cut-off value/or a second cut-off value are compared to indicate whether the subject having cancer benefits from the immunotherapy. The first cut-off value/or the second cut-off value is determined by the following steps: a statistical analysis of a correlation between the number of immune cells in a group of subjects having cancer and an expected risk of disease progression in the group of subjects having cancer is performed, and then a statistically significant value used to define the correlation is obtained.

Abstract: In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluid channel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of the second cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10 ?m to about 1 cm.

Abstract: In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.

Abstract: In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluid channel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of the second cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10 ?m to about 1 cm.

Abstract: Disclosed is a method for identifying a subject at risk of developing a recurrent or metastatic cancer, comprising detecting PD-L1+ circulating tumor cells in a blood sample, a tissue fluid sample or a specimen of the subject. Also disclosed is method for treating a cancer comprising identifying a subject having one ore more PD-L1+ circulating tumor cells by detecting PD-L1+ circulating tumor cells in a blood sample, a tissue fluid sample or a specimen of the subject, and administering a treatment to the subject.