Abstract: Lactobacillus-based probiotic compositions for inclusion in microbial-based products. In one instance, the microbial-based product takes the form of an animal feed additive. When used as a feed additive for animals, especially ruminants, and in particular cattle, the following benefits from the composition are noted: (i) improved milk yield in dairy cattle, (ii) improved milk composition in dairy cattle, (iii) increased weight gain-to-feed intake ratio in beef cattle (feed efficiency), and (vi) improved average daily weight gain. Another marked benefit of the compositions when used as an animal feed additive is inhibition of pathogenic growth including E. coli, Salmonella, Clostridium, Staphylococcus and Streptococcus.

Abstract: A bacterial composition and method that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis; (2) Enterococcus faecium (3) Pediococcus acidilactici.

Abstract: Lactobacillus-based probiotic compositions for inclusion in microbial-based products. In one instance, the microbial-based product takes the form of an animal feed additive. When used as a feed additive for animals, especially ruminants, and in particular cattle, the following benefits from the composition are noted: (i) improved milk yield in dairy cattle, (ii) improved milk composition in dairy cattle, (iii) increased weight gain-to-feed intake ratio in beef cattle (feed efficiency), and (vi) improved average daily weight gain. Both Lactobacillus strains MB101 having ATCC Accession Number PTA-121710 and MB102 having ATCC Accession Number PTA-121711 are particularly effective at producing these results. Another marked benefit of the compositions when used as an animal feed additive is inhibition of pathogenic growth including E. coli, Salmonella, Clostridium, Staphylococcus and Streptococcus.

Abstract: A probiotic, useable as a feed additive, particularly for animals, that includes live, viable beneficial bacteria strains. Because the beneficial bacteria are alive, the probiotic has a capacity to kill and inhibit the growth of pathogens such as E. coli and Salmonella. In certain select combinations, the probiotic bacteria have shown a greater than 93% reduction in E. coli and a greater than 97% reduction in Salmonella. Among other realized beneficial effects of the probiotics such as improved feed efficiency, increased milk production and enhanced pregnancy rates, pathogen inhibition has been observed in beef cattle and dairy cattle, as well as their calves. Probiotic mixtures variously containing MB101 and/or MB102 Lactobacillus animalis have shown consistent E. coli and Salmonella inhibition rates approaching 100%.

Abstract: Lactobacillus-based probiotic compositions for inclusion in microbial-based products. In one instance, the microbial-based product takes the form of an animal feed additive. When used as a feed additive for animals, especially ruminants, and in particular cattle, the following benefits from the composition are noted: (i) improved milk yield in dairy cattle, (ii) improved milk composition in dairy cattle, (iii) increased weight gain-to-feed intake ratio in beef cattle (feed efficiency), and (vi) improved average daily weight gain. Both Lactobacillus strains MB101 having ATCC Accession Number PTA-121710 and MB102 having ATCC Accession Number PTA-121711 are particularly effective at producing these results. Another marked benefit of the compositions when used as an animal feed additive is inhibition of pathogenic growth including E. coli, Salmonella, Clostridium, Staphylococcus and Streptococcus.

Abstract: A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal by providing to the animal a composition having 1×1013 cfu of Lactobacillus probiotic microorganisms per kilogram of feed. When 1×104 viable cells of the Lactobacillus probiotic are added to 1×104 viable cells of Escherichia coli in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of Escherichia coli is reduced compared to a preparation of 1×104 viable cells of Escherichia coli mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of Lactobacillus probiotic.

Abstract: Processes that produce microorganisms that can be incorporated into microbial-based products that result in high viable cell yields and shelf-stable products. Among other things, these microbial-based products inhibit pathogenic growth. In one application, the microbial-based products take the form of a supplement. In another application, the microbial-based products take the form of a food additive. When used as a supplement or food additive for animals, and in particular for cattle, the following benefits are noted: (i) increased weight gain-to-feed intake ratio (feed efficiency), (ii) improved average daily weight gain, (iii) improved milk yield, and (vi) improved milk composition by dairy cows. Both Lactobacillus strains MB101 having ATCC Accession Number PTA-121710 and MB102 having ATCC Accession Number PTA-121711 are particularly affective at producing these results, and the effects of which can be enhanced by Propionibacterium.

Abstract: A bacterial composition and method that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis; (2) Enterococcus faecium (3) Pediococcus acidilactici.

Abstract: A bacterial composition and method that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis; (2) Enterococcus faecium (3) Pediococcus acidilactici.

Abstract: A method of manufacturing an E. coli inhibiting composition that includes live Lactobacillus animalis and/or live Lactobacillus acidophilus bacterial cells. The composition can be ingested to prevent E. coli in animals, or applied as a surface treatment on things, including food items. The manufacturing method includes inoculating a bacterial fermentation medium with live Lactobacillus cells and harvesting live Lactobacillus cells at least partially between mid-log and late-log phase of growth. The harvesting of live Lactobacillus cells can occur at least partially during early-log, mid-log and late-log phase of growth. Prior to harvesting, the cell count of live Lactobacillus cells can be required to be at least 1×106 cells/ml, after which the live Lactobacillus live cell count can be concentrated to at least 5×109 cells/ml.

Abstract: Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating either a fermentation medium with enterococcus or pediococcus bacterial cells, harvesting the enterococcus or pediococcus bacterial cells at least partially during the log phases, concentrating the bacterial cells, and preserving the bacterial cells at a concentration of at least 5×109 cfu/ml.

Abstract: Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating either a fermentation medium with enterococcus or pediococcus bacterial cells, harvesting the enterococcus or pediococcus bacterial cells at least partially during the log phases, concentrating the bacterial cells, and preserving the bacterial cells at a concentration of at least 5×109 cfu/ml.

Abstract: Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with lactic acid producing bacterial cells, harvesting the lactic acid producing bacterial cells at least partially during the log phases, concentrating the lactic acid producing bacterial cells, and preserving the lactic acid producing bacterial cells at a concentration of at least 5×109 cfu/ml.

Abstract: Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with lactic acid producing bacterial cells, harvesting the lactic acid producing bacterial cells at least partially during the log phases, concentrating the lactic acid producing bacterial cells, and preserving the lactic acid producing bacterial cells at a concentration of at least 5×109 cfu/ml.

Abstract: A bacterial composition and method that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis; (2) Enterococcus faecium (3) Pediococcus acidilactici.

Abstract: Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with lactic acid producing bacterial cells, harvesting the lactic acid producing bacterial cells at mid to late log phase, concentrating the lactic acid producing bacterial cells, and preserving the lactic acid producing bacterial cells at a concentration of at least 5×109 cfu/ml.

Abstract: A dairy administered animal feed additive that promotes animal health and growth, reduces mortality and can act as an antibiotic substitute. The additive inhibits pathogen growth resulting in lower pathogen load within the animal and reduced pathogen sheading to the environment. The additive includes bacteria that inhibit E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains. The additive includes various combinations of the following pathogen-inhibiting bacteria: Lactobacillus animalis; Enterococcus faecium; and Pediococcus acidilactici.

Abstract: Composition including a pathogen inhibiting bacterial probiotic and a fructooligosaccharide (FOS) prebiotic particularly suited as a feed additive administered to juvenile animals such as calves that promotes animal health and growth, reduces scours and mortality, and can act as an antibiotic substitute. The additive inhibits pathogen growth resulting in lower pathogen load within the animal and pathogen sheading. The prebiotic-probiotic additive includes bacteria that inhibit E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, as well as the Big-Six Escherichia coli strains. The probiotic is constituted of various combinations of four pathogen-inhibiting bacteria: Lactobacillus animalis; Lactobacillus animalis; Enterococcus faecium; and Pediococcus acidilactici.

Abstract: A juvenile animal feed additive, such as for calves, that promotes animal health and growth, reduces mortality and can act as an antibiotic substitute. The additive inhibits pathogen growth resulting in lower pathogen load within the animal and reduced pathogen sheading to the environment. The additive includes bacteria that inhibit E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains. The additive includes various combinations of the following pathogen-inhibiting bacteria: Lactobacillus animalis; Enterococcus faecium; and Pediococcus acidilactici.

Abstract: A feedlot administered animal feed additive that promotes animal health and growth, reduces mortality and can act as an antibiotic substitute. The additive inhibits pathogen growth resulting in lower pathogen load within the animal and reduced pathogen sheading to the environment. The additive includes bacteria that inhibit E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains. The additive includes various combinations of the following pathogen-inhibiting bacteria: Lactobacillus animalis; Enterococcus faecium; and Pediococcus acidilactici.