Abstract: Methods and compositions for rapid detection of pathogens in biological samples, such as saliva or a nasal swab, are disclosed. Nucleic acid molecules from pathogens can be stabilized at room temperature for several days in a transport solution, followed by their amplification without prior extraction. An optimized isothermal amplification method and the amplification solution are also disclosed, allowing for reducing a threshold time for a positive sample. The methods and compositions are suitable for detection of a variety of pathogens, including enveloped viruses, such as Cytomegalovirus or SARS-CoV-2 virus, and pathogenic microorganisms.

Abstract: Methods and compositions for rapid detection of pathogens in biological samples, such as saliva or a nasal swab, are disclosed. Nucleic acid molecules from pathogens can be stabilized at room temperature for several days in a transport solution, followed by their amplification without prior extraction. An optimized isothermal amplification method and the amplification solution are also disclosed, allowing for reducing a threshold time for a positive sample. The methods and compositions are suitable for detection of a variety of pathogens, including enveloped viruses, such as Cytomegalovirus or SARS-CoV-2 virus, and pathogenic microorganisms.