Abstract: A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

Abstract: In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards, the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated.

Abstract: In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated.

Abstract: A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

Abstract: A device for generating a chemiluminescence radiation depending on the binding of at least one ligand that is contained in a sample that is to be tested to at least one receptor that is binding-specific for the ligand has a flow cell having an internal cavity. The internal cavity has a separating wall on which the at least one receptor is immobilized. The internal cavity has at least two compartments. In a first compartment solid is deposited that contains at least one peroxidase-enzyme-possessing marker for marking the ligand in lyophilized form. A second compartment contains salt-stabilized luminol and a solid substance that releases hydrogen peroxide upon contact with water.

Abstract: In a process for mixing microdroplets (2a, 2b, 2c), at least two carriers (3a, 3b) are provided whose surfaces (4a, 4b) are each structured in such a way that at least one hydrophilic surface domain (5a, 5b) is delimited by at least one hydrophobic surface domain (6a, 6b). A first microdroplet (2a, 2b, 2c) is disposed on a hydrophilic surface domain (5a) of a first carrier (3a), and a second microdroplet (2a, 2b, 3c) is disposed on a hydrophilic surface domain (5b) of a second carrier (3b). The carriers (3a, 3b), with their first and second hydrophilic surfaces (4a, 4b) facing each other, are positioned adjacent to each other and sufficiently close to each other by being moved toward each other that the microdroplets (2a, 2b, 2c) come into contact with each other.

Abstract: Disclosed is a device for detecting at least one ligand contained in a sample that is to be analyzed. Said device comprises an optical waveguide, on the surface of which at least one ligand-specific receptor is directly or indirectly immobilized. The ligand bonds to said receptor during contact therewith. The inventive device comprises at least one optical source of radiation for injecting excitation radiation into the waveguide, the radiation being used for exciting emission of luminescence radiation in accordance with the bonding of the ligand to the receptor. At least one radiation receiver is integrated into the semiconductor substrate of a semiconductor chip so as to detect the luminescence radiation. The waveguide is integrated in a monolithic manner into the semiconductor substrate or is applied thereupon as a wave-guiding layer.

Abstract: In a method for the manufacture of a microarray, surface areas of a chip are brought into contact with different aqueous solutions that contain at least one coating substance. For each of the individual surface areas, an absorbent substrate is provided and is filled with the solution that is to be brought into contact with the surface area in question. Then, for the printing of the surface area with the at least one coating substance contained in the solution, each substrate is brought into contact with the surface area in question.

Abstract: In a method for measuring the concentration of a ligand contained in a sample that is to be tested, at a plurality of test sites that are located on the surface of at least one substrate, in each case at least one receptor that is suitable upon contact with the ligand for specifically binding to said ligand, is immobilized. The sample is brought into contact for a predetermined time with the test sites in such a way that in the sample differently sized diffusion volumes are adjacent to the receptors from which at least one ligand can diffuse to the respective receptor during the predetermined time. After the predetermined time has passed, for each test site a measured signal for the number or density of the binding events at the test site is acquired.

Abstract: The invention relates to polyfunctional polymer networks comprising an assembly of cross-linked polymer subchains attached to a surface, with each polymer subchain comprising a multitude of identical or different repeating units carrying one or more functional groups which allows an interaction with a sample or probe molecule.

Abstract: The invention relates to sensor chips with multiple layers of polysiloxane with improve homogeneity.

Abstract: The invention relates to a method for detecting and/or determining the concentration of a ligand contained in a solution to be analyzed, during which a receptor, which can enter into a specific bond with the ligand, is immobilized on a semiconductor chip at a test location. In order to bind the ligand to the receptor, the solution is applied to the test location. A luminescence radiation or a color change is generated according to the binding of the ligand to the receptor. A radiation measurement signal is picked up with the aid of a radiation receiver integrated in the semiconductor chip, whereas the solution and/or an auxiliary liquid that does not contain the luminophore is in contact with the radiation receiver. While the generation of luminescence radiation is prevented and the radiation receiver is in contact with the auxiliary liquid and/or with a substitute liquid, a darkness measurement signal is measured for the radiation receiver.

Abstract: Disclosed is a device for detecting at least one luminescent substance, comprising a radiation source for emitting excitation radiation to the at least one luminescent substance. The excitation radiation is provided with at least one excitation wavelength at which the luminescent substance is excited so as to emit luminescent radiation. At least one radiation receiver is provided, which detects the luminescent radiation and is configured regarding the spectral sensitivity thereof in such a way that said radiation receiver is insensitive to the radiation emitted by the radiation source. The luminescent substance is located inside a measuring chamber that is essentially impermeable to the luminescent radiation and comprises at least one wall area which is transparent to the excitation radiation emitted by the radiation source.

Abstract: An integrated sensor array has a substrates, at least one sensor element, and a signal processing device integrated in the substrate and connected or capable of being connected with the sensor elements. A layer having a measuring surface is capable of being detachably bonded to the substrate so that a signal emitted from the measuring surface is capable of being processed by the signal processing device when the layer is bonded to the substrate. The substrate has at least one feedthrough hole abutting with the layer, which feedthrough hole is connected or capable of being connected to a vacuum generating device in order to bond the layer to the substrate by suction.

Abstract: In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated.

Abstract: In a process for manufacturing a sensor chip a support is prepared having at least one surface region in which at least one reactive group is arranged. A buffer solution is applied to the surface region for coating at least one surface region. Then, at least one receptor having binding specificity for a ligand to be detected is brought into contact with the surface coating so obtained in such a manner that said receptor binds covalently to at least one reactive group.

Abstract: Disclosed is a device for detecting at least one luminescent substance, comprising a radiation source for emitting excitation radiation to the at least one luminescent substance. The excitation radiation is provided with at least one excitation wavelength at which the luminescent substance is excited so as to emit luminescent radiation. At least one radiation receiver is provided, which detects the luminescent radiation and is configured regarding the spectral sensitivity thereof in such a way that said radiation receiver is insensitive to the radiation emitted by the radiation source. The luminescent substance is located inside a measuring chamber that is essentially impermeable to the luminescent radiation and comprises at least one wall area which is transparent to the excitation radiation emitted by the radiation source.

Abstract: The present invention relates to a linker system for activating surfaces for bioconjugation, and particularly to a linker system having a novel hydrophilic spacer group. The inventive linker system may be used for the construction of sensor chips or biochips for the detection of sample biomolecules.

Abstract: In a method for the manufacture of a microarray, surface areas of a chip are brought into contact with different aqueous solutions that contain at least one coating substance. For each of the individual surface areas, an absorbent substrate is provided and is filled with the solution that is to be brought into contact with the surface area in question. Then, for the printing of the surface area with the at least one coating substance contained in the solution, each substrate is brought into contact with the surface area in question.

Abstract: A method for covalently immobilizing glycoproteins such as immunoglobulins on solid, semi-solid or gel-like surfaces, and constructs suitable therefor, as well as surfaces and devices that comprise correspondingly immobilized glycoproteins.