Patents Assigned to MicroPhage (TM) Incorporated
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Patent number: 8455186Abstract: A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism.Type: GrantFiled: June 13, 2008Date of Patent: June 4, 2013Assignee: MicroPhage™ IncorporatedInventors: Jonathan Drew Smith, Jon C. Rees, Duane Bush, Breanna Leigh Dreiling, Maria Izzo, Breanna Christine Smith, Bernard Sportmann, Tiffany Steinmark, Richard Proctor
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Patent number: 8216780Abstract: A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×106 pfu/mL and 7×106 pfu/mL.Type: GrantFiled: June 2, 2009Date of Patent: July 10, 2012Assignee: MicroPhage (TM) IncorporatedInventors: Jonathan Drew Smith, Breanna Leigh Dreiling, Breanna Christine Smith, John H. Wheeler
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Publication number: 20110183314Abstract: A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of infecting the target microorganism to create a bacteriophage-exposed sample; and measuring the time rate of change of the amount of said bacteriophage or the change in the rate of change of the amount of said bacteriophage as an indication of the presence or absence of the target microorganism as a function of time.Type: ApplicationFiled: January 26, 2011Publication date: July 28, 2011Applicant: MicroPhage IncorporatedInventor: Jonathan D. Smith
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Publication number: 20100196877Abstract: A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism.Type: ApplicationFiled: June 13, 2008Publication date: August 5, 2010Applicant: MicroPhage IncorporatedInventors: Jonathan Drew Smith, Jon C. Rees, Duane Bush, Breanna Leigh Dreiling, Maria Izzo, Breanna Christine Smith, Bernard Sportmann, Tiffany Steinmark, Richard Proctor
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Publication number: 20090286232Abstract: A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×106pfu/mL and 7×106pfu/mL.Type: ApplicationFiled: June 2, 2009Publication date: November 19, 2009Applicant: MicroPhage™ IncorporatedInventors: Jonathan Drew Smith, Breanna Leigh Dreiling, Breanna Christine Smith, John H. Wheeler
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Publication number: 20090246752Abstract: A method for detecting one or more target bacteria in a raw sample where: 1) bacteriophage(s) specific to each target bacterium are added to the raw sample, 2) the test sample is incubated, and 3) the test sample is tested for the presence of each phage in sufficient numbers to indicate the presence of the associated target bacteria in the raw sample. In one embodiment, each phage is initially added to the raw sample in concentrations below the detection limit of the final phage detection process. In another embodiment, the parent phages are tagged in such a way that they can be separated from the progeny phage prior to the detection process. Preferred phage detection processes are immunoassay methods utilizing antibodies that bind specifically to each phage. Antibodies can be used that bind to the protein capsid of the phage. Alternatively, the phage can by dissociated after the incubation process and the sample tested for the presence of individual capsid proteins or phage nucleic acids.Type: ApplicationFiled: December 30, 2008Publication date: October 1, 2009Applicant: MicroPhage TM IncorporatedInventors: Kent Voorhees, Jon Rees, John H. Wheeler, Angelo Madonna
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Publication number: 20080286757Abstract: A sample is tested for the presence of bacteria, such as in an automatic blood culturing apparatus. If bacteria are determined to be present, a bacteriophage-based bacteria identification process is performed to identify the bacteria present. A plurality of bacteria detection processes, such as a blood culture test and Gram stain test may be carried out prior to the bacteria identification process. A bacteriophage-based antibiotic resistance test or antibiotic susceptibility test is also conducted on the sample.Type: ApplicationFiled: September 15, 2006Publication date: November 20, 2008Applicant: MicroPhage IncorporatedInventors: G. Scott Gaisford, John H. Wheeler, Jon C. Rees, Scott D. Conlin
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Publication number: 20080241819Abstract: A sample to be tested for the presence of a target microorganism is exposed to bacteriophage and conditions are provided to inhibit phage attachment to or replication in a potentially cross-reactive, non-target microorganism. The sample is incubated and assayed to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism. The inhibiting may comprise the addition of an inhibiting substance or the use of an inhibiting process. It may include inhibiting the growth of potentially cross-reactive bacteria while allowing growth of the target bacteria, selectively removing or blocking potential cross-reactive bacteria using selective binding agents or selectively destroying potentially cross-reactive bacteria.Type: ApplicationFiled: October 31, 2007Publication date: October 2, 2008Applicant: MicroPhage (TM) IncorporatedInventor: Breanna C. SMITH
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Publication number: 20070178450Abstract: A predetermined amount of parent bacteriophage capable of infecting a target microorganism is added to a sample to create a bacteriophage-exposed sample; the sample is incubated for a defined incubation time and assayed to determine the level of a bacteriophage or bacterial marker in the sample; and if the measured marker level has increased, then the initial concentration of the microorganism exceeds a specific threshold value. An antibiotic in different concentrations is added to different and separate portions of the sample and tested to determine if the bacteriophage marker is present and thereby determine the Minimum Inhibitory Concentration (MIC) of a given antibiotic. The antibiotic preferably is an antibiotic that inhibits DNA replication or protein synthesis.Type: ApplicationFiled: January 25, 2007Publication date: August 2, 2007Applicant: MicroPhage (TM) IncorporationInventors: John H. Wheeler, Jon C. Rees, Gregory S. Gaisford
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Publication number: 20050250096Abstract: A method of detecting the presence or absence of a target microorganism in a sample to be tested comprising: combining with the sample, bacteriophage capable of infecting the target microorganism to create a bacteriophage exposed sample; providing conditions to the bacteriophage exposed sample sufficient to allow the bacteriophage to infect the target microorganism to create injected bacteriophage nucleic acid, additional bacteriophage nucleic acid, intermediate bacteriophage protein, or additional bacteriophage protein; and assaying the nucleic acid or protein to determine the presence or absence of the target microorganism. The microorganism is preferably dissociated or lysed prior to the completion of the bacteriophage replication process. The sample is an insitu sample and the microorganism is isolated from the insitu matrix. The assaying comprises comparing the test nucleic acid or protein level to a reference level.Type: ApplicationFiled: June 7, 2005Publication date: November 10, 2005Applicant: Microphage, IncorporatedInventors: John Wheeler, Jon Rees, G. Gaisford