Patents Assigned to Microscience Limited
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Publication number: 20080175866Abstract: A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism; (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant; (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment; (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms; (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marType: ApplicationFiled: August 17, 2007Publication date: July 24, 2008Applicants: Imperial College Innovations Limited, Microscience LimitedInventor: David William Holden
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Patent number: 6984490Abstract: A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism; (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant; (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment; (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms; (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marType: GrantFiled: November 16, 2000Date of Patent: January 10, 2006Assignees: Imperial College Innovations Limited, Microscience LimitedInventor: David William Holden
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Patent number: 6936425Abstract: The present invention relates to vaccines, in particular, to an attenuated gram-negative cell comprising the SP12 gene locus, wherein at least one gene of the SP12 locus is inactivated, wherein the inactivation results in an attenuation/reduction of virulence compared to the wild type of said cell, and to a carrier for the presentation of an antigen to a host, which carrier is the attenuated gram-negative cell, wherein the cell comprises at least one heterologous nucleic acid molecule comprising a nucleic acid sequence coding for the antigen, wherein the cell is capable of expressing the nucleic acid molecule or capable of causing the expression of the nucleic acid molecule in a target cell.Type: GrantFiled: September 3, 1999Date of Patent: August 30, 2005Assignees: Microscience Limited, Imperial College Innovations LimitedInventors: Michael Hensel, David William Holden, Jacqueline Elizabeth Shea
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Patent number: 6812021Abstract: According to the present invention, a series of genes are identified in Group B Streptococcus, the products of which may be associated with the outer surface of the organism. The genes, or functional fragments thereof, may be useful in the preparation of therapeutics, e.g. vacaccines to immunize a patient against microbial infection.Type: GrantFiled: September 10, 2001Date of Patent: November 2, 2004Assignee: Microscience LimitedInventors: Martin John Glenton Hughes, Joseph David Santangelo, Jonathan Douglas Lane, Robert Feldman, Joanne Christine Moore, Richard James Dobson, Paul Everest, Gordon Dougan, Rebecca Kerry Wilson
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Publication number: 20040203039Abstract: The present invention relates to vaccines, in particular, to an attenuated gram-negative cell comprising the SPI2 gene locus, wherein at least one gene of the SPI2 locus is inactivated, wherein said inactivation results in an attenuation/reduction of virulence compared to the wild type of said cell, and to a carrier for the presentation of an antigen to a host, which carrier is said attenuated gram-negative cell, wherein said cell comprises at least one heterologous nucleic acid molecule comprising a nucleic acid sequence coding for said antigen, wherein said cell is capable of expressing said nucleic acid molecule or capable of causing the expression of said nucleic acid molecule in a target cell.Type: ApplicationFiled: January 23, 2004Publication date: October 14, 2004Applicants: Microscience Limited, Imperial College Innovations LimitedInventors: Michael Hensel, David William Holden, Jacqueline Elizabeth Shea
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Patent number: 6342215Abstract: A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism; (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant; (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment; (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms; (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique mType: GrantFiled: December 1, 1998Date of Patent: January 29, 2002Assignees: Imperial College Innovations Limited, Microscience LimitedInventors: David William Holden, Jacqueline Elizabeth Shea, Michael Hensel
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Patent number: 5797114Abstract: A method and apparatus for resistivity mapping of semiconductor materials by causing currents to flow in a semiconductor body and measuring resultant potentials created between pairs of surface probes. A resistivity map is produced using the information gathered.Type: GrantFiled: January 26, 1996Date of Patent: August 18, 1998Assignees: The University of Sheffield, Bio-Rad Microscience LimitedInventors: John Roberts, Ian Leslie Freeston, Richard Charles Tozer, Anthony Charles Gorvin, Ian Christopher Mayes, Francois Jean Djamdjl, Stephen Richard Blight