Patents Assigned to Middlesex Sciences, Inc.
  • Method for isolating biomolecules from a biological sample with linear polymers
    Patent number: 5599719
    Abstract: A method, composition, and kit for isolating biomolecules from a biological sample wherein the sample is mixed with a soluble, linear polymer, such as polyvinylpyrrolidone, to form a precipitate. The biomolecule of interest is found in the precipitate or is isolated from the supernatant.
    Type: Grant
    Filed: August 26, 1993
    Date of Patent: February 4, 1997
    Assignee: Middlesex Sciences, Inc.
    Inventors: James E. Woiszwillo, Fred Rothstein
  • Macromolecular microparticles and methods of production
    Patent number: 5578709
    Abstract: A macromolecular microparticle composition formed by dehydrating an aqueous macromolecule solution and crosslinking the dehydrated macromolecules with a crosslinking agent while in a liquid phase or with heat. Preferably, the dehydrating agent is a polymer mixture of polyvinylpyrrolidone and polyethylene glycol, the crosslinking reagent is glutaraldehyde, and the macromolecule is a protein, most preferably an immunoglobulin. Methods of use for research, diagnostics and therapeutics are also provided.
    Type: Grant
    Filed: March 4, 1994
    Date of Patent: November 26, 1996
    Assignee: Middlesex Sciences, Inc.
    Inventor: James E. Woiszwillo
  • Method and kit for making a polysaccharide-protein conjugate
    Patent number: 5554730
    Abstract: A method and kit for making a polysaccharide-protein Schiff base conjugate. A polysaccharide is oxidized with an oxidizing agent and combined with a protein in the presence of a macromolecular crowding agent to form a Schiff base. Preferably, the macromolecular crowding agent is a soluble linear polymer selected from the group consisting of polyvinylpyrrolidone, polyethylene glycol, dextran, nonylphenol-ethoxylates, polyvinyl alcohol, and mixtures thereof. Most preferably, the macromolecular crowding agent is a mixture of polyvinylpyrrolidone and polyethylene glycol. The microparticles or substantially dissolved microparticles are immunogenic and are useful for inducing an immune response when administered to humans or animals.
    Type: Grant
    Filed: December 23, 1994
    Date of Patent: September 10, 1996
    Assignee: Middlesex Sciences, Inc.
    Inventors: James E. Woiszwillo, Jie Di
  • Method for isolating biomolecules from a biological sample with linear polymers
    Patent number: 5525519
    Abstract: A method and composition for isolating biomolecules from a biological sample wherein the sample is mixed with a soluble, linear polymer, such as polyvinylpyrrolidone, to form a precipitate. The biomolecule of interest is found in the precipitate or is isolated from the supernatant.
    Type: Grant
    Filed: January 7, 1992
    Date of Patent: June 11, 1996
    Assignee: Middlesex Sciences, Inc.
    Inventor: James E. Woiszwillo
  • Macromolecular microparticles and method of production
    Patent number: 5484894
    Abstract: A macromolecular microparticle composition formed by dehydrating an aqueous macromolecule solution and crosslinking the dehydrated macromolecules with a crosslinking agent while in a liquid phase or with heat. Preferably, the dehydrating agent is a polymer mixture of polyvinylpyrrolidone and polyethylene glycol, the crosslinking reagent is glutaraldehyde, and the macromolecule is a protein, most preferably an immunoglobulin. Methods of use for research, diagnostics and therapeutics are also provided.
    Type: Grant
    Filed: March 4, 1994
    Date of Patent: January 16, 1996
    Assignee: Middlesex Sciences, Inc.
    Inventor: James E. Woiszwillo