Abstract: This disclosure describes biological pesticides that include biological mannosylerythritol lipids (MELs), and their application. Provided MEL-based pesticides are microbially produced by through microbial (fungal) fermentation of plant-based derivatives or plant-derived materials as a substrate. The biologically active components are obtained from multiple stage bio-processes including transformation, biochemical reaction, extraction, and other processing of raw materials. The synthesis, separation, concentration, purification, preparation of biological pesticides, and their application as a crop pathology treatment, are described. These biological pesticides may be used disease prevention and control for crops and other plants.

Abstract: Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Abstract: Provided herein are amphoteric glycolipid biosurfactants containing an amphoteric glycolipid biological surface active molecule preparation produced by acid precipitation of culture supernatant produced by sequentially culturing Pseudomonas, Candida, and Neurospora, for instance in a fermentation medium comprising a hydrophilic carbon source and a hydrophobic carbon source. Also described are amphoteric glycolipid biological surface active molecule preparations, and methods of making such preparations. The amphoteric molecules have anionic and cationic groups; example molecules include 17-L-[(2?-O-?-D-glucopyranosyl-?-D-Glucosyl)-O-]-octadecenoic acid amine-6?,6? diacetate, and 17-L-[(2?-O-?-D-glucopyranosamine-?-D-rhamnosyl)-O-]-octadecenoic acid-6?,6? diacetate. The amphoteric glycolipid biosurfactant has good compatibility with other types of surfactants, high temperature resistance and salt resistance, and is suitable for use in a variety of liquid systems.