Abstract: Apparatus for use in measuring the density and spatial distribution of macular pigment in an eye comprises a camera (4) for capturing a color image of an eye, at least one filter (36) for filtering light reaching the camera (preferably by filtering the light illuminating the eye). The filter (36) has a transmission spectrum with one peak in the region of light absorbed by the pigment and another peak in a region where no such absorption occurs. The filter increases the sensitivity of the camera to macular pigment while enabling the effect of other pigments to be reduced or eliminated. A method of measuring macular pigment involves obtaining a color image of an eye, the image having two color components each having a spectrum having a respective one of said peaks.